E11A.5(a) The following data were obtained for the absorption at 450 nm by a dye in carbon tetrachloride when using a 2.0 mm cell. Calculate the molar absorption coefficient of the dye at the wavelength employed: [dye]/(mol dm³) T/(per cent) 0.0010 0.0050 0.0100 0.0500 81.4 35.6 12.7 3.0 x 10-3
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- Consider ultraviolet radiation of wavelength 300 nm passing through 1.50 mm of a solution of absorbing substance at concentration 0.717 mmol dm-3. Transmission was found to be 61.5%. Calculate the molar absorption coefficient of the solute at 400 nm and express your answer in cm2 /mol.An amino acid on the surface of a protein was labelled covalently with 1.5-I AEDANS and another was labelled covalently with FITC. The fluorescence quantum yield of 1.5-I AEDANS decreased by 10 per cent due to quenching by FITC. What is the distance between the amino acids? (Refer to Table 17G.3 for the appropriate value of R0.)Radiation of wavelength 268 nm passed through 1.5 mm of a solution that contained benzene in a transparent solvent at a concentration of 0.080 mol dm-3 . The intensity of the radiation is reduced to 22 per cent of its initial value (so T = 0.22). (a) Calculate the absorbance and the molar absorption coefficient of the benzene. (b) What would be the transmittance through a cell of thickness 3.0 mm?
- A solution sample, in a 0.50 cm cuvette, exhibits an absorbance of A = 0.84 at lamdamax= 230 nm. Calculate the concentration of the solution if the molar absorption coefficient of the compound at 230 nm is 14 mol–1 dm3 cm–1.Radiation of wavelength 280 nm passed through 1.0 mm of a solution that contained an aqueous solution of the amino acid tryptophan at a concentration of 0.50 mmol dm-3 . The intensity of the rad iation is reduced to 54 per cent of its init ial va lue (so T = 0.54). Calcu late the molar absorpt ion coefficient and the absorbance of tryptophan at 280 nm. What would be the transmittance through a cell of thickness 2.0 mm?7) A 25.0 ml sample containing Cu gave an instrument reading of 23.6 units (corrected for a blank). When exactly 0.500 ml of 0.0287M Cu(NO3)2 Was added to the solution,the signal increased to 37.9 units. Calculate the molar concentration of Cu" in the sample +2,
- Calculate the temperature at which qvib 4.0 -.6806 X K 2.5 for nitric oxide. The vibrational wavenumber isỹ = 1876 cm-1.At 520 nm with a 1.00 cm cell a 8.24 ppm solution of KMnO4 has a transmittance of 0.126. What is the molar absorptivity of KMnO4 at 520 nm?The quantum yield of light-induced chemical reactions (called photochemical reactions) measures the efficiency of the process. The quantum yield, ϕ, is defined as:ϕ=numberofreactioneventsnumberofphotonsabsorbed . Suppose a quantum yield for the reaction CH3X→CH3+Xis ϕ= 0.95. A cuvette containing a solution of CH3Xis irradiated with 280-nmwith a power of 885 mWfor 10.0 minutes. Part A Assuming total absorption of the light by the sample, what is the maximum amount (in moles) of CH3Xthat breaks apart?Express your answer to two significant figures. nothingmolCH3Xm o l C H 3 X actual
- P17G.3 Dansyl chloride, which absorbs maximally at 330 nm and fluoresces maximally at 510 nm, can be used to label amino acids in fluorescence microscopy and FRET studies. Tabulated below is the variation of the fluorescence intensity of an aqueous solution of dansyl chloride with time after excitation by a short laser pulse (with I, the initial fluorescence intensity). The ratio of intensities is equal to the ratio of the rates of photon emission. t/ns 5.0 10.0 15.0 20.0 II. 0.45 0.21 0.11 0.05 (a) Calculate the observed fluorescence lifetime of dansyl chloride in water. (b) The fluorescence quantum yield of dansyl chloride in water is 0.70. What is the fluorescence rate constant?P17G.3 Dansyl chloride, which absorbs maximally at 330 nm and fluoresces maximally at 510 nm, can be used to label amino acids in fluorescence microscopy and FRET studies. Tabulated below is the variation of the fluorescence intensity of an aqueous solution of dansyl chloride with time after excitation by a short laser pulse (with I, the initial fluorescence intensity). The ratio of intensities is equal to the ratio of the rates of photon emission. t/ns 5.0 10.0 15.0 20.0 0.45 0.21 0.11 0.05 (a) Calculate the observed fluorescence lifetime of dansyl chloride in water. (b) The fluorescence quantum yield of dansyl chloride in water is 0.70. What is the fluorescence rate constant?distinguish between the circumstances for the use of nRln(V2/V1) and Cvln(T2/T1) in calculating change in S