You are measuring the concentration of an unknown protein sample, and the absorbance at 595 nm (or A595) of your unknown is greater than that of the highest-concentration standard solution. It is not advisable to simply extrapolate the line of the standard curve to calculate the concentration of the unknown In this scenario, what would be the best strategy to measure the concentration of your unknown (instead of extrapolating from the standard curve)?

Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
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Chapter1: Chemical Foundations
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You are measuring the concentration of an unknown protein sample, and the absorbance at 595 nm (or A595) of your unknown is greater than that of the highest-concentration standard solution. It is not advisable to simply extrapolate the line of the standard curve to calculate the concentration of the unknown

In this scenario, what would be the best strategy to measure the concentration of your unknown (instead of extrapolating from the standard curve)?

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