ConcentraticTransmittancAbsorbance% (with : variable % (with : variable % (with : variable110010960.017730800.096940730.136650610.2147Transmittance, Absorbance vs Concentration100806040201020304050Concentration (% (with yellow LED))Display Curve Fit UncertaintiesTransmittance Curve:Absorbance Curve:y = Ax + By = Ax + B(with yellow LED)A: -0.773 ± 0.0584 With yellow LED)A: 0.00417 + 0.000474 4 with yellow LED)yellow LED)B : 102 ± 1.86 % (with yellow LED)B :-0.0152 0.0151 % (with yellow LED)RMSE : 2.42% (with yellow LED)RMSE : 0.0197% (with yellow LED)r: -0.992r: 0.9812.LOTransmittance (% (with yellow LED)), Absorbance (% (n . Describe your results. What is the shape of the graph of absorbance vs concentration? What does this tell us about crystal violetsolutions?

Concentratic Transmittanc Absorbance ... % (with variable % (with : variable % (with variable 1 100 ... 2 10 96 0.0177 3 30 80 0.0969 4 40 73 0.1366 ... 50 61 0.2147 ... Transmittance, Absorbance vs Concentration 100 80 60 40 20 10 20 30 40 50 Concentration (% (with yellow LED)) Transmittance (with yellow LED)), Absorbance (% (n

Concentratic Transmittanc Absorbance ... % (with variable % (with : variable % (with variable 1 100 ... 2 10 96 0.0177 3 30 80 0.0969 4 40 73 0.1366 ... 50 61 0.2147 ... Transmittance, Absorbance vs Concentration 100 80 60 40 20 10 20 30 40 50 Concentration (% (with yellow LED)) Transmittance (with yellow LED)), Absorbance (% (n

Organic Chemistry: A Guided Inquiry

2nd Edition

ISBN:9780618974122

Author:Andrei Straumanis

Publisher:Andrei Straumanis

ChapterL1: Infrared Spectroscopy

Section: Chapter Questions

Problem 2CTQ

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Similar questions

Which is TRUE for spectroscopy of a mixture? A. The absorbance for a mixture at a particular wavelength is the sum of the absorbances for the components that absorb at the particular wavelength. B. Spectrophotometers can differentiate between mixture components that absorb at the same wavelength. C. Each component in a mixture has the same molar absorptivity at the same wavelength. D. The concentration for each component in a mixture is easily calculated by least squares for guesses of each component when the individual spectra are well resolved.
Kiko wants to determine the concentration of an unknown solution which produces a green solution. However, the spectrophotometer he uses doesn't have a function to determine the analytical wavelength of the solution. What is the most probable analytical wavelength he can use for the experiment? Refer to the color wheel below.
A protein’s concentration can be determined spectrophotometrically by mixing it with an indicator called a Bradford reagent and measuring the amount of light absorbed by the protein. Use the plot below to determine the mass of protein (in micrograms, μμg ) in 100.0 mL of the solution if its absorbance is 0.398. A. 52 B. 3.1 C. 2.4 D. 0.52 E. 0.031
Why must solutions with high concentrations be diluted prior to analysis via Beer's Law? A. The relationship between absorbance and concentration is not linear at high concentrations. B. The detector will reach its detection threshold. C. The photon source is too weak to provide accurate results. D. There is no need to work with dilute concentrations; any concentrations will work. E. The molar absorptivity of a compound is dependent on its concentration.
Cite one research article that used an IR spectrophotometer in their study and discuss how they used the instrument to generate their data and how they used the data of IR in answering their hypothesis.
True or false? A. Phosphorescence occurs at higher energy then fluorescence. B. Fluorescence occurs at longer wavelengths than phosphorescence. C. Fluorescence occurs at shorter wavenumbers than phosphorescence.
a. When the light source is monochromatic, Beer's Law holds true. b. Beer's Law establishes a relationship between the concentration of a sample and its absorbance, wherein absorbance is the ratio of light leaving the sample to light entering it. c. For solutions that have more than one absorbing species, Beer's Law holds true even if the analytes of interest don't interact.
Why do we need to prepare a standard curve before we can use Beer's Law to determine the concentration of a solution? O We don't have to create a standard curve so long as we choose the appropriate wavelength. Because the standard curve tells us the absorbance of our sample Because the standard curve is used to determine the extinction coefficient Because the standard curve indicates what wavelength to set the spectrophotometer to
10. In what ways can a refractive index be used to characterize a liquid sample? 11. Describe how to adjust the light for measurement in the Abbe Refractometer
4. A cuvette containing a colored solution in the path of a laser beam absorbs 12% of the incident light. If a second identical cuvette of the same solution is added to the beam path, what percentage of the incident light will reach the detector? A. More than 24% B. Less that 24% C. 24%
Which of the following are true regarding phosphorescence and fluorescence? Read through all before making your one selection. A. Phosphorescence occurs at a lower frequencies than fluorescence B. Fluorescence occurs at longer wavelengths than phosphorescence. C. Fluorescence occurs at shorter wavenumbers than phosphorescence. D. B and A E. A and C F. All the above are true G. None of the above are true
How will you use a solution of known concentration of one of the food dyes to determine the relationship between concentration and absorbance? Be sure to explain how you determined the wavelength for your analysis.