3. (a) The following are the results from subjecting the lectin to SDS-PAGE: Band / Molecular Length 210 100 67 48 32 Lectin Weight (kDa) of Gel Migration Distance 7.2 1.8 2.5 3.2 3.9 4.7 3.5 (cm) Determine the molecular weight of the lectin. Show all workings.

3. (a) The following are the results from subjecting the lectin to SDS-PAGE: Band / Molecular Length 210 100 67 48 32 Lectin Weight (kDa) of Gel Migration Distance 7.2 1.8 2.5 3.2 3.9 4.7 3.5 (cm) Determine the molecular weight of the lectin. Show all workings.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter27: Gas Chromatography

Section: Chapter Questions

Problem 27.18QAP

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Flame Atomic Absorption Spectroscopy (FAAS) method is selected due to its wide applicability for metal ion analysis in drinking water samples. Provide appropriate discussion to determine the concentration of selected heavy metals (Cu and Cr) in the unknown tap water sample.
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write a summary of each of the techniques for measuring vacancyconcentrations, noted therein. (1) Measurements of the quenched-in residual electricalresistivity of aluminium rapidly cooled from temperatures be-low about 800 K with initial quenching rates of 2 × 106 K s−1give the enthalpy of formation of monovacancies H F1V =(0.65 ± 0.01) eV.(2) Comparison of the quenching data with high-temperature differential-dilatometry data gives the monova-cancy formation entropy SF1V = (0.76 ± 0.4)kB (kB =Boltzmann’s constant) and the low-temperature resistivity perunit concentration of vacancies ρ1V = (1.9 ± 0.1) μm.(3) The binding enthalpy of divacancies in Al is H B2V =(0.17 5 ± 0.02 5) eV. This is in agreement with earlier ex-perimental determinations by Doyama and Koehler 48) and byLevy et al. 49, 50) but in marked contrast to the claim of Carlinget al., 23) based on computations with the generalized gradi-ent approximation, that in Al H B2V is zero or even negativeand therefore the…
I) Diamond crystallises in a face-centred cubic structure with a = 3.5625 Å. Calculate the d-spacing and peak position of the first allowed peak (i.e. the peak with the lowest hkl value that meets the selection rules) using a wavelength of 0.71073 Å. II) A solvothermal synthesis gave rise to a mixture of two crystalline phases: one phase consists of 50-65 μm octahedral crystallites while the other phase consists of 150-180 μm hexagonal plates. Identify a separation technique that would likely be effective in separating these materials.
(a) A sample of body serum is to be analysed for sodium by flame emission spectroscopy. 1.00cm3 aliquot of serum was pipetted into each of two 50.0 cm3 volumetric flasks. The firstflask was diluted to volume with deionised water. The absorbance of this first solution was0.350. To the second flask 10.0 cm3 of a 25.0 ppm sodium standard was added and theflask made up to volume with deionised water. The absorbance of this second solution was0.720.(i) Calculate the concentration of the sodium in the body serum in mg dm–3
One common way to determine phosphorous in urine is to treat the sample after removing the protein with molybdenum (VI) and then reducing the resulting 12-molybdophosphate complex with ascorbic acid to give an intense blue-coloured species called molybdenum blue. The absorbance of molybdenum blue can be measured at 650 nm. A 24-hour urine sample was collected, and the patient produced 1122 mL in 24 hours. A 1.00 mL aliquot of the sample was treated with Mo (VI) and ascorbic acid and diluted to a volume of 50.00 mL A calibration curve was prepared by treating 1.00 mL aliquot of phosphate standard solution in the same manner as the urine sample. The absorbances of the standards and the urine sample were obtained at 650 nm and the following results were obtained. ppm, P aBSORBANCE (A) 1.003.00 0.230 2.00 0.436 3.00 0.638 4.00 0.848 Urine sample 0.518 ffind the slope and intercept, then graph it.
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Flame Atomic Absorption Spectroscopy (FAAS) method is selected due to its wide applicability for metal ion analysis in drinking water samples. Discuss how can we determine the concentration of selected heavy metals (Cu and Cr) in the unknown tap water sample.
standard polystyrene samples in tetrahydrofuran solution were obtained [M. Kolinsky The following data on intrinsic viscosities and GPC peak elution volumes at 25°C for and J. Janca, J. Polym. Sci., Chem. Ed., 12, 1181 (1974)]: Mwx 10-3 [n] (cm /g) 206.7 Ve (cm³) 867 411 173 98.2 51 19.85 10.3 5.0 125.0 67.0 43.6 27.6 14.0 8.8 5.2 149 157 177 234 253.5 186.5 Construct an elution volume calibration curve for poly(vinyl bromide) in tetranyaro- füran at 25°C, given that the MHS constants for this system are K = 1.59×10-4 cm2/g and a = 0.64 [A. Ciferri, M. Kryezewski, and G. Weil, J. Polym. Sci., 21, 167 (1958)], and calculate the weight average molecular weight of the polymer. 199.5 219