. Carbonic anhydrase of erythrocytes (M, 30,000) has one of the highest turnover numbers known. It catalyzes the reversiblehydration of CO₂.H₂O + CO₂H₂CO3This is an important process in the transport of CO₂ from the tissues to the lungs. If 3 µg of pure carbonic anhydrase catalyzesthe hydration of 0.10 g of CO₂ in 1 min at 37 °C at Vmax, what is the turnover number (kcat) of carbonic anhydrase?Keat=3.79 ×10-¹4IncorrectImin

The turnover number or kcat of an enzyme is defined as the maximum number of substrate molecules…

Answered: Carbonic anhydrase of erythrocytes (M,…

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter21: Heparin Infusion Calculations

Section: Chapter Questions

Problem 2SST

See similar textbooks

Similar questions

Carbonic anhydrase of erythrocytes (Mr 30,000) has one of the highest turnover numbers known. It catalyzes the reversible hydration of CO₂. H₂O + CO₂ 2 H₂CO3 This is an important process in the transport of CO₂ from the tissues to the lungs. If 5 µg of pure carbonic anhydrase catalyzes the hydration of 0.35 g of CO₂ in 1 min at 37 °C at Vmax, what is the turnover number (kcat) of carbonic anhydrase? Kcat 2.0466 ×107 Incorrect min-1
Derivatioh The equilibrium between dissolved CO2 and carbonic acid can be represented by k12 H+ + HCO3 H2CO3 k21 k13 || k31 К23 К 32 CO2 H2O Show that k23 d[CO2] = (k31 + k32)[CO2] - (k13 + 3 )tH* I[HCO3¯1 K dt where K = [H+][HCO3¯]/[H2CO3]. Step 1 of 4 The equilibrium between dissolved CO2 and carbonic acid can be represented by k12 H+ + HCO; H,CO3 k21 k13 k31 k23 К 32 CO2 H2O d[CO2] corresponding to the given equilibrium. (Assume that H2O is incorporated in the appropriate equilibrium constants. Use the following as necessary: [CO2], [H+], Give the expression for dt [HCO3 ], [H2C03], k12, k13, k21, k23, k31, and k32-) k23 H HCO3 d[CO2] (k31 + k32)[ CO2] - (kK13 + k dt Submit
. Oxygen binding by the hemocyanin of the shrimp Callianassa has been measured. Using the following data, prepare a Hill plot and determine (a) Pso, (b) h (the Hill coefficient), and (c) the minimum number of oxygen binding sites on the protein molecule. Poz (mm Hg) Yoz Po, (mm Hg) Yo2 1.1 0.003 136.7 0.557 7.7 0.019 166.8 0.673 10.7 0.035 203.2 0.734 31.7 0.084 262.2 0.794 71.9 0.190 327.0 0.834 100.5 0.329 452.0 0.875 123.3 0.487 736.7 0.913
The various forms of vitamin K Function as cofactors for enzymes with a key role in blood coagulation O Function as cofactors in the blood coagulation pathway, and as chemical antioxidants Have different functions; one form is an enzyme cofactor, and another is a potent antioxidant O All serve as antioxidants
Measurements of oxygen binding by whok human blood, at 37 °C, at pH 7.4, and in the presence of 40 mm Hg of CO, and normal physiological lkvels of 2,3-BPG (5 mmol/L of cells), give the following: Po, (mm Hg) % Saturation (=100 x Yo) 10.6 10 19.5 30 27.4 50 37.5 70 50.4 85 77.3 96 92.3 98 (a) From these data, construct a binding curve, and estimate the percent oxygen saturation of blood at (1) 100 mm Hg, the approximate partial pressure of O, in the lungs, and (2) 30 mm Hg, the approximate partial pressure of Oz in venous blood. (b) Under these conditions, what percentage of the oxygen bound in the lungs is delivered to the tissues? (c) Using the data in Figure 7.27, repeat the calculation of part (b) if the pH drops to 6.8 in capillaries but goes back to 7.4 as CO, is unloaded in the lungs.
A 42 yr old woman is not in shape but still decides to run a marathon. During the event, she collapses and is brought to the ER. Arterial blood gas analysis shows PO2 of 100 mmHg (normal), PCO2 of 42 mmHg (normal), and pH of 7.30 (low). Further evaluation reveals high anion-gap metabolic acidosis. Increased activity of which of the following biochemical pathways is the basis of the clinical findings? a. ETC b. Citric acid cycle c. Anaerobic glycolysis d. Beta-oxidation of fatty acids e. Mitochondrial oxidative phosphorylation
calculate the actual free energy of hydrolysis of ATP, delta Gp in the erythrocytes of a new species. The standard free-energy of hydrolysis of ATP is also -30.5kJ/mol in this species, but the concentrations in this specie's erythrocytes are 0.00775 mM ATP, 0.00027 mM ADP and 0.00418 mM Pi. Assume the pH is 7.0 and the body temperature of this species is 43.0oC. Calculate your answer as kJ/mol to two decimal places.
Acid phosphatases are an important group of enzymes that can be detected in human bloodserum. Under slightly acidic conditions (pH 5.0), this group of enzymes can hydrolyzebiological phosphate esters as follows:R-O-P-O3-2 + H2O -----> R-OH + HO-P-O3-2Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver,and prostrate gland. The enzyme from the prostrate gland is clinically important because anincreased activity in the blood is frequently an indication of cancer of the prostrate gland.Tartrate ion can strongly inhibit the phosphatase from the prostrate gland, but not acidphosphatases from other tissues. How can you use the information above to develop a specific procedure for measuring the activity of the acid phosphatase of the prostrate gland in humanblood serum?
"Hemoglobin can be in either an oxy- or deoxy- state at a given pH. Given the appropriate pKa values, calculate fraction protonated at a given pH" How would I do this?
(No more than one page). Explain why quaternary structure is necessary for cooperativity and allosteric regulation. Use hemoglobin as an example. Include the various allosteric effectors that influence oxygen binding.
Characterizing Glycolysis List the reactions of glycolysis that a. are energy consuming (under standard-stale conditions), b. are energy yielding (under standard-state conditions), c. consume ATP. d. yield ATP e. are strongly influenced by changes in concentration of substrate and product because of their molecularity. f. are at or near equilibrium in the erythrocyte (see Table 18.2).
Understanding the Mechanism of Hemolytic Anemia Genetic defects in glycolytic enzymes can have serious consequences for humans For example, defects in the gene for pyruvate kinase can result in a condition known as hemolytic anemia. Consult a reference to learn about hemolytic anemia, and discuss why such genetic defects lead to this condition.

SEE MORE QUESTIONS

Recommended textbooks for you

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:

9781305143531

Author:

CURREN

Publisher:

Cengage

Human Physiology: From Cells to Systems (MindTap …

Biology

ISBN:

9781285866932

Author:

Lauralee Sherwood

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:

9781305143531

Author:

CURREN

Publisher:

Cengage

Human Physiology: From Cells to Systems (MindTap …

Biology

ISBN:

9781285866932

Author:

Lauralee Sherwood

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

SEE MORE TEXTBOOKS