Calculate the isoelectric point of the tetrapeptide Ser-Leu-Phe-Pro at pH 7.0
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Calculate the isoelectric point of the tetrapeptide Ser-Leu-Phe-Pro at pH 7.0
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- 2. You have 15mL of a 0.3M solution of His-Lys-Leu-Asp adjusted to pH=9. You titrate your solution with 10mL of 0.2M HCl. Relevant pka values are 2.1,3.8,6.0,9.8 and 10.5. A) Draw the structure of the tetrapeptide that predominates in solution before you add any HCl. B) Calculate the new pH value of the solution after the addition of HCI. C) You digest this tetrapeptide with trypsin at pH=( and apply the products to an anion exchange column. Write the amino acid sequence of the products that are produced. If you gradually add HCl to the column, in what order will these products elute, if at all?Calculate the overall net charge of the following polypeptide at pH = 2.0 Gly-Pro-Glu-Asp-Leu-His-lle-Gln-Asn-Phe A) +1.761. Draw the tetrapeptide Met-Ala-Thr-Thr at a ph of 7? 2. Draw the tetrapeptide Met-Ala-Thr-Thr at a ph of 12?
- 1. Choose the proper structure of the predominant form of L-M-L-P-D-T at pH 5. Provide a short explanation. 2. Choose the proper structure of the predominant form of L-A-L-Y at pH 11. Provide a short explanation.Determine the net charge on the following pentapeptide "Lys-Ala-His-Asp-Ser" at: a. pH 1.0 b. pH 10.180mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pl. The solution is then titrated with 0.2M HCI to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCl needed to reach each relevant pKa value and equivalence point(s). Relevant pka values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.
- Draw the structure of the pentapeptide Gln-Trp-His-Glu-Tyr that would predominate in acqueous solution at pH=10.3. (Relevant pKa values are: 2.2,4.3,6.0,9.8 and 10.1)Given Poly-L-Lysine and Epsilon Poly-L-Lysine, which polymer(s) can easily dissolve in a saline solution with a pH=7 and at room temperature.4. A. Draw the structure of the following peptide sequence at pH 2.0: ASP-ALA-THR-LYS-GLY B. What is the net charge of this pentapeptide at pH 2.0?
- 1. Cysteine is an important amino acid that stabilizes the structure of peptides and proteins by the formation of disulfide bond with another cysteine residue. Illustrate the titrimetric profile of cysteine and calculate its isoelectric pH. [Hint: The sulfhydryl group is titratable] 2. The tripeptide Glu-Pro-Arg (EPR) which is a product of Lactobacillus casei fermentation of milk was found to have potent blood pressure lowering properties. Draw the structure of the tripeptide, give its systematic name, show its titration profile, and determine its isoelectric pH.1. Draw the structural formula of the amino acid valine that predominates in solution at each of the following pH values. А. рH 3D7.0 В. рH %3D 12.0 С. рH %3D 2.0 2. Draw the structural formula of the amino acid phenylalanine that predominates in solution at each of the following pH values. А. рH%3D 3.0 В. рH %3D 10.0 С. рH%3D 7.0Determine the net charge (to the nearest integer) on the following peptide at pH 5 AND pH 12. Estimate the isoelectric point (pl) for this peptide: H2N-Leu-Gly-Lys-Glu-COOH Assume the pK,s for functional groups are: alpha-amino 6. alpha-carboxy sidechain-amino (Lys) 10.5 sidechain-carboxy (Glu) 4.2 O Net charge at pH 5 and 12 is 0 and -2, respectively, pl =6.6 O Net charge at pH 5 and 12 is 0 and -2, respectively, pl =3.1 O Net charge at plH 5 and 12 is +2 and -1, respectively, pl =7.5 O Nct charge at pH 5 and 12 is +1 and -1. respectively,. pl -4.5 2.