A total of 7.21 g of dry mixture with a protein content of 89.2% (w/w) determined by Kjeldahl analysis was dissolved in 88.54 g of sterile water and centrifuged to purify the protein in the mixture. After centrifugation, a total of 5.95 g of precipitated pellet was formed, and the protein content in pellet was determined as 72.2% by spectrophotometric Lowry analysis. Assume that pellet is not a hygroscopic material and does not absorb any water. Determine the following: a. the protein concentration in the supernatant layer after centrifugation in % w/w b. the nature of amino acids in the protein structure. What's the possible explanation?
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- 2.1The number of bacteria in the samples need to be quantified. The laboratory uses a specificprotocol where the dilution factor on the spread plates must be:10-6; 10-7; 10-8Describe how you would prepare the required dilution series using the least amount of dilutionwater. 2.2 Calculate the amount of bacteria in the sample from the following results (show the steps in the calculation): Dilution factor Count10-6 27110-7 3510-8 213. 4. Draw a picture of a ten-fold serial dilution. Please use 10ul (1ml) of initial sample into 9ml of solution. Show a picture of what a serial dilution would look like up through ten- thousand-fold. You can draw this and take a picture.How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.
- 1) You have been asked to make up four 1.5% agarose gels at 30 ml each. Ethidium Bromide is to be added at 0.5 ug/ml and you have a bottle at 10ug/ml. Write down step-by-step protocol of how to proceed. 2. How would you make up one liter of 1X TAE buffer using a 25X stock?You have a sample at 50 ng/ul and you would like to load 400ng of this sample on a lane of an agarose gel. You also have TE buffer as diluent and 6x loading dye. Your total sample should be 12ul. Calculate the amounts of each reagent necessary to prepare this sample for gel loading.1. (2.5 pts) A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as diagrammed below. Each dilution tube contained 400 ul of diluent and 100 ul was transferred into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes. a. What is the dilution between each tube shown in the diagram below? Express your answer as a ratio. b. What is the total dilution of tube number 5? Express your answer as a ratio. c. What is the concentration of viable bacteria in the original culture? Express your answer using scientific notation and the units CFU/ml. d. What is the concentration of viable bacteria in tube number 2? Express your answer using the units CFU/ml. e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would you expect to see after the plate was incubated? 1 2 3 5 423 80 13 Number of colonies
- After doing the preliminary studies on redcrest protein extract, Tighnari proceeded with its characterization and analysis proper. He purified the crude protein extract through ammonium sulfate precipitation (40-60%) and gel filtration chromatography. Shown below are the results of the analyses. ||||| 250 kDa Crude Protein Extract 200 kDa 100 kDa 80 kDa 40 kDa 20 kDa ||| || Partially Purified Protein Extract Purified Protein Extract Figure D.1. Electrophoretogram obtained after NATIVE PAGE of the provided protein extracts. PAGE Analysis: 1. Based on Figure D.1., the series of purification procedures led to the isolation of two major redcrest proteins, COL and LEI. Determine the molecular weight of COL and LEI if the results of GFC showed that COL eluted out of the column first.10 If3 ml of culture is diluted by adding 9 ml of water and the absorbancy reading of the diluted culture is 0.082, what is the actual absorbancy?Ex. 5-4 Catalase 1) In the Catalase test (5-4), if bacteria was added to hydrogen peroxide on the slide, it might produce a false result. 2) Explain why adding bacteria to the hydrogen peroxide on a slide rather than adding the hydrogen peroxide to the bacteria on the slide might give a false result? 3) Would the false result be a false positive or would the false result be a false negative?
- Calculate the amount of protein (in mg) in Sample 1 if the measurement at A280 = 0.636, taking into account the dilution factor as per question 6 (100ul) and the total volume of extract as per question 4 (140ml). Give your answer as a number with no decimal places.AE, 62, Male Wt: 75 kg, Ht: 150 cm Orders: Penicillin G Potassium 10,000,000 Units Sterile water for injection qs Make a 250,000 U/mL solution. The package insert states If Penicillin G Potassium 10,000,000 Units is reconstituted with 44 mL sterile water, a solution of 200,000 U per mL will be obtained. What volume of sterile water would you use to reconstitute the penicillin G potassium in order to make the ordered solution?What is Zeta Sedimentation Ratio? Differentiate Wintrobe from Westergreen regarding the accuracy of the test in a tabulated order. Why do men have lower ESR values than women?