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- 8. Match the following. A. Killing all bacteria on medical equipment via autoclaving 1. Sanitization B. Swabbing an injection site with alcohol prior to inoculation 2. Disinfection C. Application of triple antiviotic ointment to a topical wound 3. Degerming D. Use of phenol based product to clean a toilet 4. Sterilization E. Washing eating utensils in the dishwasher 5. Antisepsis 6. Chemical F. Incineration of Biohazard waste G. Filtration of heat labile liquids 7. Biological 8. Physical H. Using bacteriophages as a treatment I. Using Nitric Oxide to sterilize petri plates 9. MechanicalILLUSTRATIONS: 1. Sterilizers seen in the laboratory and their parts.1. Write briefly about Metabolism of drugs ( just 5 lines ) 2. Write briefly about Polyethylene glycol ( just 3 lines ) 3. The levigating agent , explain about its role in Suppositories manufacturing 4. Write in details about types of gel 5. Write in details about fusion method in ointment preparation
- 1. What is the key ingredient of hand sanitizers?  include a image of the chemical structure of the ingredient and explain how it works 2. What did Ignacio Semmelweis discover? Describe the situation and what he recommended to the medical community Define the following: 1. Asepsis: 2. Disinfectant: 3. Antiseptic: 4. Sanitization: 5. Bacteriostatic: 6. Bactericidal: 7. Thermal death point (TDP): 8. Thermal death time (TDT): 9. Decimal Reduction Time (DRT):35. There is a mystery and an unusual bacterial disease in Contra Costa County. Researchers as well as physicians conclude that it must be controlled before it goes out of hand. They also find that the pathogen has a extended generation time. What antibiotic do you suggest to use? Group of answer choices Rifamycin Penicillin Quinolones ( such as ciprofloxacillin) Tetracycline Sulfa drug and trimethoprim combination 36. Which of the following best describes action of the ciprofloxacillin in prokaryotes ? Group of answer choices inhibits RNA polymerase, consequently no RNA synthesis in bacteria interferes with DNA replication by randomly nicking the DNA enhances DNA gyrase activity in killing UTI pathogens binds and modifies elongation factor ( ADP ribosylates) inhibits prokaryotic translation by binding to 50S subunits and arresting translocation inhibits DNA gyrase; prevents snapping back of the separated strands at replication fork
- Item 1. Describe the BEST method of sterilization or treatment to reduce pathogens to safe level for each of these 5 items. 2. Explain why this is the best method and why it is BETTER than other possible methods. (Specific treatments were mentioned in lecture for each item.) Plastic milk containers 1. Preserving low-acid vegetables to kill Clostridium botulinum endospores. 2. Liquids contaminated with prions (list BOTH methods of sterilization discussed in lecture) 3. Skin 4. Media with heat sensitive nutrients 5.3. Describe the below listed methods for evaluating disinfectants and antiseptics. 2.1. Phenol coefficient 2.2. Use-dilution test 2.3. Kelsey-Sykes capacity test 2.4. In-use test1. Which of the following methods is the best way to sterilize the apparatus used in a surgical operation? A. Pasteurization B. Incineration C. Boiling in water D. Autoclaving E. Disinfect with 75% alcohol
- 1. Describe the three different type of hemolysis that are observed on blood agar.2. What is a selective medium?3. What is a differential medium?4. Which media can be used to isolate E. coli samples from contaminated lettuce?5. Which two media would be used to identify a sample taken from a patient with suspected gonorrhea?6. Would you be able to grow a sample obtained from a patient's wound (suspected to be infected with MRSA) on EMB? Explain.7. What is the color or TSI for Salmonella?8. What is a fastidious organism?1) Which of the following apply to alcohols? Mark ALL that apply: - disinfect items that are soaking in it - denature proteins (50-90%) - at 50% and higher disrupt lipid membranes of cells - can be used as an antiseptic wipe - one of the best things to put on a cut or scrape 2)What would you use when you know the causative microbe of the disease in your patient? -A narrow spectrum antimicrobial-A broad spectrum antimicrobial -Neither, you would let the patient heal by themselves -Both broad spectrum and narrow spectrum antimicrobial6. Gram negative bacteria are naturally resistant to chloramphenicol. True or False.
