Where are proton gradients formed? Within what structures are they seen in chloroplasts How do the structures help them to maintain a gradient?
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For the following questions, choose one to discuss: chloroplast/photosynthesis State at the outset which one you will discuss.
A) What role do proton gradients play in the process of photosynthesis proton gradients allow
B) Where are proton gradients formed? Within what structures are they seen in chloroplasts How do the structures help them to maintain a gradient?
C) Explain where and how the chloroplast or mitochondria uses passive transport and active transport to complete photosynthesis or
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- In chloroplasts, the light reactions power the creation of ATP via chemiosmosis. In relation to this process, which of the following is true? a) ATP synthase breaks ATP down into ADP, creating energy to fuel chemiosmosis. b) Oxygen is used as a source of electrons to replace those lost in photosystem II. c) Chemiosmosis during the light reactions is also used in the reduction of NADPH and FADH2. d) As excited electrons fall back down to a lower energy state, they create a proton gradient that is used to fuel the phosphorylation of ADP.Thylakoids were isolated from chloroplasts and incubated in the dark in an acidic solution (pH 4) to equilibrate the pH. After 30 minutes, the thylakoids were transferred to a basic solution (pH 8) and kept in the dark. Will this system produce ATP? Explain. Will this system produce G3P? Explain.Consider how the Hill Reaction might be used to investigate how the wavelength of light influences the rate electrons move in the electron transport chain of the chloroplast. a) Design an experiment to compare the effects of blue light and green light on the Hill Reaction. (Assume that you have filters that you can attach to your light source to control the wavelength of light.) b) Based on your knowledge of photosynthesis, how would you expect the color of the light to effect the movement of electrons? c) Draw a line graph of your expected results.
- Identify the chemical basis for ApH and AY across the chloroplast thylakoid membrane by dragging the descriptions to their targets. Be sure to notice that the upper arrow iindicates ApH and the lower arrow indicates ΔΨ. ATP synthase complex H+ N ADP + P₁ Light energy ATP H*N Photosystem I/II- Chloroplast N side Aus PN ApH T + Thylakoid membrane HTp H+p Lumen Stroma P side Proton circuit A B High H concentration Low positive charge High positive charge Low H+ concentration Within the image, identify the types of proton translocation by dragging each label to its target. O XH₂ 2H+ + Z 2 H* ZH₂ O XH₂ Z 2H+ ZH₂ 2H+ C A B Proton pump Redox loopConsider the structures and functions of mitochondria and chloroplasts. For each of the statements below, identify which part(s) of the chloroplast or mitochondrion (identified by letters in the figure below) are described. Some answers may include more than 1 letter. In those cases, separate the letters by a single space (eg. c g) Electron transport chains are located in ------ Photosystem I and II are located in ------- Ubiquinone is located in ------- NADPH is produced in ------ Pyruvate oxidation takes place in ------ ATP is produced in ------ NAD+ is produced in ----- High H+ concentration is produced in ----- O2 is produced in ----- RuBP is produced in ------You will create a slide show/ Canva/ Website on the flow chart of photosynthesis phases .Your poster should include, but is not limited to:○ The overall equation is at the top.○ A diagram of a chloroplast which indicates where each reactant is used and whereeach product is synthesized. ○ All intermediary steps were studied in the light-dependent reactions and light-independent reactions, in the form of a flow chart(try to add details around them), using appropriate terminology. ● You will also submit a detailed explanation of your flow chart, using appropriateterminology.
- 2.) A.) Explain how electron transfer leads to ATP biosynthesis in the light reactions of photosynthesis. You must give details about how electron flow builds a pH gradient and a description of the compartments in the chloroplast in relation to pH. Also, compare the ETC of the mitochondrion with the ETC of the light reactions for both the source and destination for electrons. B.) Would ATP biosynthesis happen if ionophores, which would create holes in the thylakoid membrane and allow uncontrolled passage of charged molecules such as protons between compartments, are provided to the chloroplast? Why or why not? C.) How would you modify Complex I of the electron transport chain to decrease ATP yield from Step 3 of β-oxidation? As part of this, you must explain why there is a difference in ATP yield between NADH and FADH 2 AND give a specific change to the ETC that would decrease ATP yield from Step 3Provide a simplified schematic presentation of (non-cyclic) photophosphorylation. Clearly indicate the location and direction of electron and proton flow as well as organelle compartments. (You can also draw the Z-scheme but then you have to include ADP phosphorylation).In photosynthesis, contrast the light reactions with the light independent reactions, in terms of goals, inputs, and outputs of each. Spatially where do they take place (which parts of the chloroplast)? (Optional, for further discussion: Could there be light reactions without light-independent reactions, or visa-versa?)
- Photosynthesis and aerobic cellular respiration both rely on electron transport chains to generate ATP. Which of the following does not correctly identify similarities and differences in the ETCs of these processes? a) Electrons delivered to the ETC are used to generate a proton gradient across the membrane b) In photosynthesis, the facilitated diffusion of protons across the membrane generates ATP and glucose molecules; in cellular respiration, this process generates ATP c) In photosynthesis, electrons are delivered to the ETC by NADPH; in cellular respiration, electrons are delivered to the ETC by NADH and FADH2 d) In prokaryotes, active transport moves protons across the cell’s plasma membrane during photosynthesis and cellular respiration(c) Compare the differences between oxidative phosphorylation and photophosphorylation by redrawing (if necessary) and completing the table given below: Table 1: Comparison of oxidative phosphorylation and photophosphorylation Oxidative phosphorylation Photophosphorylation Organelle Source of electrons Final electron acceptor Source of energy Role of ATP1. A) For the schematic of a chloroplast shown in the image attached, match the major input and output molecules of photosynthesis to the letters shown. Molecules: CO2, H2O, O2, sugar A: B: C: D: B) In the image attached, the light reactions are shown to take place in the thylakoid. Where specifically do these reactions take place? C) Where does the Calvin cycle take place?