Genetics: Analysis and Principles
6th Edition
ISBN: 9781259616020
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 7.4, Problem 1COMQ
Summary Introduction
Introduction:
The process of introduction of foreign DNA (deoxyribonucleic acid) from a bacterial cell to another bacterial cell with the help of a viral vector, is known as transduction. Such kind of gene transfer is a type of horizontal gene transfer.
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The contents of a plaque may not always contain a clonal population of phage if:
a. Identical phages from a neighboring plaque diffuse into the original plaque
b. The bacteria run out of resources too quickly and limit phage growth
c. Different phages from a neighboring plaque diffuse into the original plaque
d. All plaques on the plate originated from a pure phage culture
Some strains of Escherichia coli bacteria have acquired the ability to produce the harmful Shiga toxin, normally produced by Shigella dysenteriae. Which statements best explain why this occurs? Mark all that apply.
A. conjugation between two different species of bacteria allowed both strains of bacteria to express a virulence factor
B. transformation resulted in expression of a virulence factor
C. horizontal gene transfer between different species of bacteria
D. transduction of a specific gene resulted in expression of new traits
In Hershey and Chase’s experiment investigating which biological molecule carries genetic information, they found that phage DNA with radiolabeled phosphorus was retained in bacterial cells after transduction. To conclude that DNA is the genetic material, what assumption did they have to make?
(photo linked)
A.
Protein does not enter the bacterium during transduction.
B.
DNA does not stay in the media after transduction.
C.
All of the phage DNA was radiolabeled.
D.
Bacteria can be transduced by phage.
Chapter 7 Solutions
Genetics: Analysis and Principles
Ch. 7.1 - 1. A form of genetic transfer that involves the...Ch. 7.2 - 1. A bacterial cell with an F factor conjugates...Ch. 7.2 - 2. Which of the following is a type of plasmid?...Ch. 7.3 - 1. With regard to conjugation, a key difference...Ch. 7.3 - 2. In mapping experiments, ______ strains are...Ch. 7.4 - Prob. 1COMQCh. 7.4 - Cotransduction may be used to map bacterial genes...Ch. 7.5 - Prob. 1COMQCh. 7.5 - Prob. 2COMQCh. 7.6 - 1. Which of the following is an example of...
Ch. 7 - 1. The terms conjugation, transduction, and...Ch. 7 - Prob. 2CONQCh. 7 - If you mix together an equal number of F+ and F...Ch. 7 - What is the difference between an F+ and an Hfr...Ch. 7 - 5. What is the role of the origin of transfer...Ch. 7 - 6. What is the role of sex pili during...Ch. 7 - Prob. 7CONQCh. 7 - Prob. 8CONQCh. 7 - Prob. 9CONQCh. 7 - 10. What is cotransduction? What determines the...Ch. 7 - Prob. 11CONQCh. 7 - Prob. 12CONQCh. 7 - Describe the steps that occur during bacterial...Ch. 7 - Prob. 14CONQCh. 7 - Prob. 15CONQCh. 7 - Antibiotics such as tetracycline, streptomycin,...Ch. 7 - Prob. 1EQCh. 7 - 2. In the experiment of Figure 7.1, Lederberg and...Ch. 7 - Explain how a U-tube apparatus can distinguish...Ch. 7 - Prob. 4EQCh. 7 - 5. In a conjugation experiment, what is meant by...Ch. 7 - In your laboratory, you have an F strain of E....Ch. 7 - 7. As mentioned in question 2 of More Genetic...Ch. 7 - An Hfr strain that is hisE+ and pheA+ was mixed...Ch. 7 - Acridine orange is a chemical that inhibits the...Ch. 7 - Prob. 10EQCh. 7 - Prob. 11EQCh. 7 - Lets suppose a new strain of P1 phage has been...Ch. 7 - If two bacterial genes are 0.6 minute apart on the...Ch. 7 - 14. In a cotransduction experiment involving P1,...Ch. 7 - Prob. 15EQCh. 7 - Prob. 16EQCh. 7 - 1. Discuss the advantages of the genetic analysis...Ch. 7 - Prob. 2QSDC
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- Phage M13 has often been used for Select one: O a. Specialized transduction b. generalized transduction O c. phage display d. conjugationarrow_forwardWhat happens when T2 phages are grown with radioactive phosphorus? a. Their DNA becomes radioactive. b. Their proteins become radioactive. c. Their DNA is found to be medium density in a centrifuge tube. d. They are no longer able to transform bacterial cells. e. They transfer their radioactivity to E. coli chromosomes during infection. ...Explain your answer.arrow_forwardSome scientists are trying to engineer bacteriophageto treat bacterial infections in humans when theinfections do not respond to chemical antibiotics.a. What possible advantages might phage therapyhave over antibiotic therapy?b. Describe potential difficulties that would need tobe overcome for phage therapy to succeed.c. How might researchers best confront the issue thatbacterial cells could become resistant to bacteriophage just as they could to antibiotics?arrow_forward
- In Hershey and Chase’s experiment investigating which biological molecule carries genetic information, they found that phage DNA with radiolabeled phosphorus was retained in bacterial cells after transduction. To conclude that DNA is the genetic material, what assumption did they have to make? A. Protein does not enter the bacterium during transduction. B. DNA does not stay in the media after transduction. C. All of the phage DNA was radiolabeled. D. Bacteria can be transduced by phage.arrow_forwardPhage conversion in which viruses add genes to a bacterial cell can be considered to be a form of a. standard inheritance. b. horizontal gene transfer. c. vertical gene transfer. d. parasitism.arrow_forwardDuring the late stage T4 phage infection, the phage DNA is synthesized by Select one: а. the DNA polymerase encoded by T4 genome. the ADP-ribosylated DNA polymerase encoded by E. coli genome. O c. the ADP-ribosylated DNA polymerase encoded by T4 genome. d. the DNA polymerase encoded by E. coli genome.arrow_forward
- Explain the importance of the following features in conjugating donor bacteria: a. the origin of transfer b. the conjugation pilus c. homologous recombination d. the relaxosome e. relaxase f. T strand DNA g. pillin proteinarrow_forwardIndicate whether each statement applies to the lytic or lysogenic cycle of phage A, or to both. 1. The host cell is lysed. (Click to select) v 2. The genetic material of the phage is copied. (Click to select) v 3. The genetic material of the phage is integrated into the bacterium's chromosome. (Click to select) v 4. Two ends of the phage DNA become covalently attached to one another. (Click to select) v 5. The integrase gene is turned on.: (Click to select) v (Click to select) lytic lysogenic botharrow_forwardConjugation involves which of the following: a. transfer of plasmids among bacteria b. sexual reproduction of Eukaryotes c. uptake of free DNA from the environment d. integration of a virus into a chromosomearrow_forward
- Some scientists are trying to engineer bacteriophageto treat bacterial infections in humans when theinfections do not respond to chemical antibiotics.a. What possible advantages might phage therapyhave over antibiotic therapy?arrow_forward1- which of the following can be used for treating/combating bacterial infections a. targeting spikes or attachment molecules b. phages c. reverse transcriptase inhibitors d. agent that turns ON gene expression e. agents that promote phage conversion 2- can DNA replication result in genetic change? explain asap pleasearrow_forwardGeneralized transduction can transfer: please explain the answer a.any DNA from the host genome b.only phage-specific DNA sequences c.genes flanked by insertion elements d.only genes near the site of integrationarrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license