Concept explainers
Interpretation
The Isoelectric point (pI) of proline is to be calculated. In an electrophoresis experiment, will proline migrate at
Concept introduction:
Electrophoresis is a common way of separating a mixture of amino acid. In electrophoresis, any ion that has a net positive charge, at that pH, will migrate toward the negatively charged terminal whereas any ion having a net negative charge will migrate toward the positively charged terminal.
An amino acid’s pI is the average of its two pKa values associated with the zwitterion- the pKa value corresponding to the proton transfer reaction in which the zwitterion is a product, and the pKa value corresponding to the proton transfer reaction in which the zwitterion is a reactant.
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Organic Chemistry: Principles and Mechanisms (Second Edition)
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