Microbiology with Diseases by Body System (5th Edition)
5th Edition
ISBN: 9780134477206
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Chapter 6, Problem 11CT
Suppose you perform a serial dilution of 0.1-ml sample from a liter of culture medium as illustrated in Figure 6.23. The 10–3 plate gives 440 colonies, and the 10–4 plate gives 45 colonies. Calculate the approximate number of bacteria in the original liter.
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You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs.
You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution scheme
Using a dilution series is a way to transfer the very small volumes of original culture necessary to get countable colony forming units.
If you suspect your culture of bacteria has 59.62*10^9 cells per mL, how many mL of original culture would you want to end up on the plate to produce 68 cfu?
Express your answer in scientific notation rounded to two decimal places.
Eg. 0.01001 would be 1.00*10^-2
You must include trailing zeros
0.0000040001 would be reported as 4.00*10^-6
There must always be two decimal places, even if they are zeros
Canvas expects one digit before the decimal point
0.40*10^-5 would be marked incorrect for 0.0000040001
A 0.00001 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 223 colonies of bacteria grow on the petri dish.
Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using exponential notation.
Since only 0.1 mL is put on the plate, this counts as an extra dilution!!!
Any time less than 1 mL is transfered, a dilution is being performed.
Any time more than 1 mL is transfered, a concentration is being performed.
Chapter 6 Solutions
Microbiology with Diseases by Body System (5th Edition)
Ch. 6 - Why should cardiac nurses and respiratory...Ch. 6 - Cavities Gone Wild Five-year-old Daniel appears to...Ch. 6 - Why do clinical laboratory scientists keep many...Ch. 6 - Boils in the Locker Room For several weeks,...Ch. 6 - Some students transfer some gunk from a 2-week-old...Ch. 6 - Which of the following can grow in a Petri plate...Ch. 6 - In the laboratory, a sterile inoculating loop is...Ch. 6 - Superoxide dismutase _____________. a. causes...Ch. 6 - The most reactive of the four toxic forms of...Ch. 6 - Microaerophiles that grow best with a high...
Ch. 6 - Prob. 6MCCh. 6 - Organisms that preferentially may thrive in icy...Ch. 6 - Prob. 8MCCh. 6 - Which of the following terms best describes an...Ch. 6 - In a defined medium, ______________. a. the exact...Ch. 6 - Which of the following is most useful in...Ch. 6 - Which of the following methods is best for...Ch. 6 - A Coulter counter is a(n) ________________. a....Ch. 6 - Lyophilization can be described as ___________. a....Ch. 6 - Quorum sensing is _____________. a. the ability to...Ch. 6 - Prob. 1FIBCh. 6 - Prob. 2FIBCh. 6 - Prob. 3FIBCh. 6 - Prob. 4FIBCh. 6 - Prob. 5FIBCh. 6 - Fill in the Blanks 6. Cells that shrink in...Ch. 6 - Fill in the Blanks 7. Obligate ________ exist in...Ch. 6 - Prob. 8FIBCh. 6 - Fill in the Blanks 9. Microbes that reduce N2 to...Ch. 6 - Fill in the Blanks 10. A student observes a...Ch. 6 - Fill in the Blanks 11. Chemolithotrophs acquire...Ch. 6 - Prob. 1VICh. 6 - Prob. 2VICh. 6 - High temperature affects the shape of particular...Ch. 6 - Support or refute the following statement:...Ch. 6 - Explain quorum sensing, and describe how it is...Ch. 6 - Why must media, vessels, and instruments be...Ch. 6 - Why is agar used in microbiology?Ch. 6 - What is the difference between complex media and...Ch. 6 - Draw and label the four distinct phases of a...Ch. 6 - If there are 47 cells in 1 l of sewage, how many...Ch. 6 - Prob. 9SACh. 6 - Prob. 10SACh. 6 - Explain the differences among photoautotrophs,...Ch. 6 - Contrast the media described in Tables 6.3 and 6.4...Ch. 6 - How does a chemostat maintain a constant...Ch. 6 - A scientist describes an organism as a...Ch. 6 - Pasteurization is a technique that uses...Ch. 6 - Two cultures of a facultative anaerobe are grown...Ch. 6 - Some organisms require riboflavin (vitamin B2) to...Ch. 6 - A scientist inoculates a bacterium into a complex...Ch. 6 - How can regions within biofilms differ in their...Ch. 6 - A scientific article describes a bacterium as an...Ch. 6 - Prob. 8CTCh. 6 - Prob. 9CTCh. 6 - Starting with 10 bacterial cells per milliliter in...Ch. 6 - Suppose you perform a serial dilution of 0.1-ml...Ch. 6 - How might the study of biofilms benefit humans?Ch. 6 - The filamentous bacterium Beggiatoa gets its...Ch. 6 - Given that Haemophilus ducreyi is a...Ch. 6 - Examine the graph in Figure 6.3. Note that the...Ch. 6 - Prob. 16CTCh. 6 - Using the terms in Figure 6.8a, describe the...Ch. 6 - Prob. 18CTCh. 6 - Prob. 19CTCh. 6 - Prob. 20CTCh. 6 - Prob. 21CTCh. 6 - Prob. 22CTCh. 6 - Viable plate counts are used to estimate...Ch. 6 - Using the following terms, fill in the following...
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- How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume? suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10^-1 dilution of the entire culture. Explain how you would do this. Show your calculations.arrow_forwardYou perform a ten-fold serial dilution of a bacterial culture to determine the number of colony forming units (CFU) per mL in the culture. You then do a plate count with these growth results (no. of colonies): 1:10: too many to count; 1:100 too many to count; 1:1,000: 126 colonies; 1:10,000 14 colonies; 1:100,000: no growth The number of CFU per mL in the original culture was: 1 ml 1 ml 1 ml 1 ml Original inoculum Dilutions 1,000 126 9 ml broth in each tube 126,000 10,000 140,000 1:10 1 ml 1:100 1 ml 1:1000 1 ml 1 ml 1:10,000 1 ml 1:100,000 1 mlarrow_forwardIf 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies, calculate thenumber of cells per ml of the original culturearrow_forward
- A bacterial strain has a generation time of 30 min, how long would it take an exponentially growing culture to increase from a titre of 5 x 103 cells per ml to 4 x 109 cells per ml? How many generations would have elapsed? Show all of your calculations. G = t/n to calculate generation time (i.e. for population to double) During the growth of the culture, periodic observations of the cells under the microscope have revealed the occurrence of typical rod-shaped cells. However, after further incubation, they appeared to change their morphology to form small oval or spherical-shaped structures. What do these structures likely represent? And what are the likely factors contributing to this observation? Explain what growth phase this culture will be in.arrow_forwardYou were asked to prepare a dilution series of a bacterial culture where only 3 tubes will be used (all with 5 mL total solution). Draw a schematic diagram to make tube 1 have a 10-2 dilution, tube 2 have 10-3 dilution, and tube 3 to have 10-5 dilution You were instructed to dilute an antibiotic solution 1/10, redilute 1/25, and again 1/50, then you need to make 100mL of each dilution. How would you go about preparing this dilution series? (Present your answer in a schematic diagram)arrow_forwardDesign a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL bacterial concentration. show solutionarrow_forward
- Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If we can order 5 milligrams of fibronectin for $871.00, how much would it cost to have enough to use every well of the 24-well culture plate?arrow_forwardWhat would the final concentration of a bacteria culture be if 2.7 x 106 cells/ml were diluted 8.2 x 10-2? What would the initial concentration of a bacteria culture be if the final concentration was 3.7 x 102 cells/ml and the total dilution was 4.6 x 10-4?arrow_forwardA 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 278 colony forming units grow on the plate. How many bacteria are in a single mL of the original culture? Express your answer to two decimal places using scientific notation. In scientific notation 540 would be written as 5.40*10^2. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transferred, a dilution is being performed. Any time more than 1 mL is transferred, a concentration is being performed. Include the trailing zero so there are two decimal places Canvas expects a single digit before the decimal point. 5.40*10^2 is how Canvas expects 540 to be formatted in scientific notation 54.00*10^1 would be marked wrong. 0.54*10^3 would be marked wrong. A 10-5 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the…arrow_forward
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