Principles of Instrumental Analysis
7th Edition
ISBN: 9781305577213
Author: Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher: Cengage Learning
expand_more
expand_more
format_list_bulleted
Concept explainers
Videos
Textbook Question
Chapter 33, Problem 33.5QAP
Give the advantages and disadvantages of sequential injection analyzers compared to traditional flow Injection analyzers.
Expert Solution & Answer
Want to see the full answer?
Check out a sample textbook solution
Students have asked these similar questions
The amount of caffeine in an analgesic tablet was determined by HPLC using a
normal calibration curve. Standard solutions of caffeine were prepared and analyzed
using a 10-µL fixed-volume injection loop. Results for the standards are summarized
in the following table.
Concentration of Standards
Signal
(arbitrary units)
(ppm)
50.0
8354
100.0
16925
150.0
25218
200.0
33584
250.0
42002
The sample was prepared by placing a single analgesic tablet in a small beaker and
adding 10 mL of methanol. After allowing the sample to dissolve, the contents of the
beaker, including the insoluble binder, were quantitatively transferred to a 25-mL
volumetric flask and diluted to volume with methanol. The sample was then filtered,
and a 1.00-mL aliquot was transferred to a 10-mL volumetric flask and diluted to
volume with methanol. When analyzed by HPLC, the signal for the caffeine was
found to be 21469. Report the number of milligrams of caffeine in the analgesic
tablet.
analyte
concentration(C)(mg/ml)
injection volume (ul)
elution time (time)
peak DAD signal(mAU)
caffeine
1
1
4.67
302.85
aspartame
5
1
7.53
15.83
benzoic acid
1
1
8.14
89.98
saccharin
1
1
1.91
84.86
mixture(add everything above with 1:1:1:1 ratio)
1
4.47
69.58
How to get the concentration of the mixture in this case?
Which of the following describes a gradient elution process?
stationary phase has varying concentration
stationary phase has static concentration
mobile phase has varying concentration
mobile phase has static concentration
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.Similar questions
- What are the advantages and disadvantages of volumetric and gravimetric analysis?arrow_forwardWhat are the differences between systematic and random errors and how do they effect accuracy and precision? In what circumstances would you use standard addition (versus a normal calibration curve) to determine the amount of an analyte in a sample? A urine sample, containing analyte Z is analysed by the standard addition method where 5 mL of the original sample was mixed with increasing amounts of a Z standard and each solution diluted to a volume of 50 mL prior to analysis. A plot of the final concentration of the standard in each of the 50 mL samples (x axis) versus The measured signal from the analysis of each 50 mL sample (on y axis) produced a straight line with the general equation: y = 44.72x + 4.06 what was the final concentration of Z in the 50 mL standard addition sample? what was the initial concentration of Z in the original urine sample?arrow_forwardThe internal standard method compensates for errors that affect both the analyte and the reference. True Falsearrow_forward
- Define Extraction in chemistry ?arrow_forwardWhat are method blanks and method detection limit used in analytical chemistry? Explain thoroughlyarrow_forwardIf an analytical method relies on a measurable property of an analyte that can be correlated with the amount of the said analyte, what type of analytical method is it? Oxidative methods Instrumental methods Blank Correction methods Classical methodsarrow_forward
- What is the retention factor (Rf) of the most polar and the least polar spot present in the standard ink?arrow_forwardEnumerate the differences between a serological and a volumetric pipette and enumerate the uses, advantages, and limitations of a glass versus a plastic volumetric pipette.arrow_forwardWhat are the Pros and Cons of Standard Addition Method versus External Calibration Methods?arrow_forward
- Color BEFORE adding Color AFTER adding Intensity of color AFTER adding FeCl3 Test Tube # FeCl3 FeCl3 #1 (salicylic acid) clear lilac 100% #2 (commercial aspirin A) clear slightly pink 50% #3 (commercial aspirin B) clear lilac 95% #4 (aspirin from your clear pink 20% synthesis above) #5 (control) clear clear 0% Based on this data and the melting point of Aspirin, how pure is the synthesized product?arrow_forwardPick the HETP (height equivalent to a theoretical plate) listed below that represents the most efficient column. O1 mm/plate O 1 micrometer/plate 1 cm/plate 1 m/platearrow_forwardRetention time is the time taken for the mobile phase to reach the detector. True Falsearrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning
Principles of Instrumental Analysis
Chemistry
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Cengage Learning
Molecular spectroscopy; Author: Vidya-mitra;https://www.youtube.com/watch?v=G6HjLIWvCQo;License: Standard YouTube License, CC-BY