Microbiology: An Evolving Science (Fourth Edition)
Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 2.3, Problem 2TQ
Summary Introduction

To review:

The parameters determining that the cell under microscopic observation is hollow or is simply out of focus.

Introduction:

The observation of a bacterial specimen depends upon the relative distance of the object with the focal point. The resolution of the specimen or the object also changes because of an alteration of the focal point. Maximum resolution of the specimen can be obtained from the focal point.

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If the diameter of the field of view in your microscope is 2 mm under low power, and one Bacillus cell is 2 µm long, how many Bacillus cells would it take to reach all the way across this field of view? If yeast cells measure 10 µm in length, how many yeast cells would it take to reach across this same field of view?
You are looking at a larger cell (approximately 50 micrometers) under a microscope, and see a nucleus and membrane-bound organelles. What type of cell can you confirm you are observing?
Let’s say you wanted to look at cells of Bacillus cereus, which are rod-shaped cells that are about 4 µm long. If you were observing B. cereus with a microscope using the high-power lens, how big would the cells appear to be when you look at them?
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