Campbell Biology
12th Edition
ISBN: 9780135188743
Author: Urry
Publisher: PEARSON
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Chapter 20, Problem 4TYU
A paleontologist has recovered a bit of tissue from the 400-year old preserved skin of an extinct dodo (a bird). Tocompare a specific region of the DNA from a sample vvith DNA from living birds, which of the following would be most useful for increasing the amount of dodo DNA available for testing?
- (A) SNPanalysis
- (B) Polymerase chain reaction (PCR)
- (C) electroporation
- (D) gel electrophoresis
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Given the situation: A paleontologist has recovered a tiny bit of organic material fromthe 400-year-old preserved skin of an extinct dodo. She would like to compare DNA from the sample with DNA from living birds.Which of the following would be most useful for increasing theamount of DNA available for testing?
Option a.) restriction fragment analysis
Option b.) polymerase chain reaction
Option c.) electrophoresis
Which of the following best describes the complete sequence of steps occurring during every cycle of PCR?
I-The primers hybridize to the target DNA.
II-The mixture is heated to a high temperature to denature the double stranded target DNA.
III-Fresh DNA polymerase is added.
IV-DNA polymerase extends the primers to make a copy of the target DNA.
A) II, I, IV.
B) I, III, II, IV.
C) III, IV, I, II.
D) III, IV, II.
E) II, III, IV.
Explain briefly(a) PCR(b) Restriction enzymes and DNA(c) Chitinase
Chapter 20 Solutions
Campbell Biology
Ch. 20.1 - Prob. 1CCCh. 20.1 - DRAW IT One Strand of a DNA molecule has the...Ch. 20.1 - What are some potential difficulties in using...Ch. 20.1 - VISUAL SKILLS Compare Figure 20.7 with Figure...Ch. 20.2 - Prob. 1CCCh. 20.2 - Prob. 2CCCh. 20.3 - Based on current knowledge, how would you explain...Ch. 20.3 - Prob. 2CCCh. 20.3 - Prob. 3CCCh. 20.4 - What is the advantage of using stem cells for gene...
Ch. 20.4 - Prob. 2CCCh. 20.4 - Prob. 3CCCh. 20 - Describe how the process of gene doning results in...Ch. 20 - What useful Information is obtained by detecting...Ch. 20 - Describe how, using mice. a researcher could carry...Ch. 20 - What factors affecf whether a given genetic...Ch. 20 - In DNA technology, the term vector can refer to...Ch. 20 - Which of the following tools of DNA technology is...Ch. 20 - Prob. 3TYUCh. 20 - A paleontologist has recovered a bit of tissue...Ch. 20 - Which of the following is not true of cDNA...Ch. 20 - Expression of a cloned eukaryotic gene in a...Ch. 20 - Which Ii of the following sequences in...Ch. 20 - Prob. 8TYUCh. 20 - MAKE CONNECTIONS Looking at Figure 20.15, what...Ch. 20 - DRAW IT You are cloning an aardvark gene, using a...Ch. 20 - EVOLUTlON CONNECTION Ethical considerations aside,...Ch. 20 - Prob. 12TYUCh. 20 - Prob. 13TYUCh. 20 - Prob. 14TYU
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- Which of the following ingredients does not belong in a PCR reaction? (A) DNA polymerase B Primer Ligase D) DNTPS E) ddNTPs F) Endonucleasearrow_forwardScientists have reportedly isolated short fragments of DNA from fossilized dinosaur bones hundreds of millions of years old. The technique used to isolate this DNA is the polymerase chain reaction, which is capable of amplifying very small amounts of DNA a millionfold. Critics have claimed that the DNA isolated from dinosaur bones is not purely of ancient origin, but instead has been contaminated by DNA from present-day organisms such as bacteria, mold, or humans. What precautions, analyses, and control experiments could be carried out to ensure that DNA recovered from fossils is truly of ancient origin?arrow_forward(A) After three cycles of PCR, how many DNA molecules are present that correspond precisely to the desired amplification product? (B) What about after 5 cycles. Assume that we started with one molecule in each case, and that the reaction is perfectly efficient.arrow_forward
- a) (iii) (iv) Polymerase chain reaction (PCR) is a technique that enables multiplication of specific DNA sample at minute amount to millions or billions of copies at a short time span. (i) Figure 1 indicates the chemicals added into the PCR reaction tube prior to the addition of thermostable DNA polymerase. Do you agree with the list? Justify your answer. DNA template Buffer (containing Tris-HCI, KCI, Mg2+) ddNTP Forward primer Reverse primer Figure 1 If TA cloning is planned to be carried out after amplification of the gene, which thermostable DNA polymerase will you select and the reason for your selection? Why is the optimal annealing temperature vital in this technique? Explain how this temperature (too high or too low) will affect the efficiency of this reaction. If the primers you purchased possessed the following information: Number of Guanine: 4 Number of Adenine: 3 Number of Thymine: 4 Number of Cytosine: 5 Calculate the melting temperature of this primer and estimate the…arrow_forwardRecombinant DNA is:(a) DNA that is produced when genes from one kind oforganism are introduced by lateral transfer into the ge-nome of another kind of organism(b) DNA that is destroyed by endonucleases(c) DNA amplified by the polymerase chain reaction(d) DNA that is rich in the nitrogenous base guanine(e) All of the abovearrow_forwardTo amplify a section of DNA using the polymerase chain reaction (PCR), all you need to load into the tube is 1) a buffer solution, 2) the DNA you want to amplify, 3) some DNA nucleotides, 4) a polymerase (like Taq polymerase), and O an RNA polymerase a set of forward and reverse primers some phospholipids for a cell membrane some ribosomesarrow_forward
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