Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Chapter 2, Problem 1CA

A wet mount may be used for quick examination of fecal samples. Is this most useful for identifying protozoan or bacterial parasites? Briefly explain.

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What precautions must be observed when collecting fecal specimen for examination? What  portion of the fecal specimen should be sampled Which of the preservatives are best used for observation of protozoan morphology? Why is it necessary to examine loose, diarrheic, watery or mucoid stool within 30 minutes of passage? Why is contamination of stool with water, urine or soil contraindicated?
What is the proper collection time period for a fecal fat specimen? 1) 24 hours 2) 48 hours 3) 72 hours 4) 96 hours
Answer the following questions (not more than 5 sentences/question).  Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test.  Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.
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