Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 19.4, Problem 2MQ
- What types of environments limit the application of GFP?
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Where is GFP localized within a cell and why?
Which of the IgG subclasses is most efficient at activating complement?
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Chapter 19 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 19.1 - Describe the enrichment strategy behind...Ch. 19.1 - Why is sulfate (So42) added to a Winogradsky...Ch. 19.1 - What is enrichment bias? How does dilution reduce...Ch. 19.1 - Why do the results of a direct enrichment of an...Ch. 19.2 - What is a pure culture and why is obtaining one...Ch. 19.2 - How does the agar dilution method differ from...Ch. 19.2 - What criteria serve to demonstrate that a culture...Ch. 19.3 - How might you isolate a morphologically unique...Ch. 19.3 - What is meant by high-throughput in culturing...Ch. 19.3 - What feature of high-throughput culturing relieves...
Ch. 19.4 - How does viability staining differ from stains...Ch. 19.4 - What types of environments limit the application...Ch. 19.4 - Why is it incorrect to say that the GFP is a...Ch. 19.4 - Prob. 1CRCh. 19.5 - What structure in the cell is the target for...Ch. 19.5 - FISH and CARD-FISH can be used to reveal different...Ch. 19.5 - Why is CARD-FISH more suitable than FISH for...Ch. 19.6 - What could you conclude from PCR/DGGE analysis of...Ch. 19.6 - What surprising finding has come out of many...Ch. 19.6 - How has next-generation sequencing technology...Ch. 19.6 - QWhich method, ARISA or T-RFLP, would provide more...Ch. 19.7 - Prob. 1MQCh. 19.7 - What are the advantages and disadvantages of...Ch. 19.7 - Why might a microarray be superior to using...Ch. 19.8 - Prob. 1MQCh. 19.8 - How do environmental genomic approaches differ...Ch. 19.8 - Prob. 3MQCh. 19.8 - Prob. 1CRCh. 19.9 - Prob. 1MQCh. 19.9 - If a large pulse of organic matter entered the...Ch. 19.9 - Q What are the major advantages of radioisotopic...Ch. 19.10 - What is the simplest explanation for why lunar...Ch. 19.10 - What is the expected isotopic composition of...Ch. 19.10 - How might exchange of metabolites among members of...Ch. 19.10 - Will autotrophic organisms contain more or less...Ch. 19.11 - How could NanoSIMS be used to identify a...Ch. 19.11 - Prob. 2MQCh. 19.11 - How does MAR-FISH link microbial diversity and...Ch. 19.11 - Q What can MAR-FISH tell you that FISH alone...Ch. 19.12 - How can stable isotope probing reveal the identity...Ch. 19.12 - What key method is required to do genomics on a...Ch. 19.12 - Prob. 3MQCh. 19.12 - How would you use cytometric cell sorting to...Ch. 19 - Design an experiment for measuring the activity of...Ch. 19 - You wish to know whether Archaea exist in a lake...Ch. 19 - Design an experiment to solve the following...Ch. 19 - Design a SIP experiment that would allow you to...
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- Which of the following is one of the family of immunoglobin like proteins that mediate Ca+2?a) N-CAMb) Integrinc) Cadherind) Selectinarrow_forwardWhich of the enzymatic fragments are able to bind antigen?arrow_forwardWhich is the most appropriate example of a product formed by an indirect form of gene therapy? a )Stem cells b) Insulin c )Antigen substitution d )Platelet inhibitorsarrow_forward
- Warm autoimmune hemolytic anemia, WAIHA, frequently have antibody specificity of: Question 6 options: A) anti-P B) anti-I C) anti-i D) anti-earrow_forwarda. Compare and contrast the Sec and Tat systems in bacteria. b. Why do Gram-negative bacteria need one- and two-step secretion systems? What is the difference between these two systems? c. There has been a recent outbreak of a bacterial infection in humans that causes high fever, vomiting, and diarrhea. You identify the cause of this infection as a pathogenic Gram-negative bacterium called Bacterium Q, or, more specifically, the Q toxin secreted by this bacterium. Make a hypothesis about the type of secretion system this pathogen has and explain how this secretion system works to secrete Q toxin.arrow_forwardFor Western Blot detection of specific proteins, the secondary antibody directly binds to: A) The antigen-binding sites of the primary antibody B) The protein of interest on the membrane C) The Fc domain of the primary antibodyarrow_forward
- Briefly describe three ways that antibody diversity is increased.arrow_forwardWhat are the mechanisms by which the attenuator forms when tryptophan levels are high and the antiterminator forms when tryptophan levels are low?arrow_forwardExplain why IgG subclasses is most efficient at activating complement?arrow_forward
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Chapter 5 Microbial Metabolism; Author: Heather Davis;https://www.youtube.com/watch?v=wH_HrsfDWZw;License: Standard Youtube License