You are a medical technologist assigned in a blood banking laboratory and you are tasked to prepare a 5% red cell suspension using 0.3 mL of pRBCs. Find: a. total volume of red cell suspension b. total volume of diluent 2. If the total volume of red cell suspension is 5mL, how many percentage of red cell suspension is prepared using 0.1 mL pRBC?
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Solve the following:
1. You are a medical technologist assigned in a blood banking laboratory and you are tasked to prepare a 5% red cell suspension using 0.3 mL of pRBCs.
Find:
a. total volume of red cell suspension
b. total volume of diluent
2. If the total volume of red cell suspension is 5mL, how many percentage of red cell suspension is prepared using 0.1 mL pRBC?
3. Calculate the total volume of the diluent that you are going to use if you have prepared a 3% red cell suspension using 0.5 mL of pRBC.
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Solved in 2 steps
- Phenylephrine and nafazolin solutions are used as decongestants to relieve nasal congestion. Your patient has been given the following prescription below. You need to prepare 100 g of this nasal spray and make it isotonic. 1. There is an available 25% w/w Phenylephrine solution in the pharmacy. How many g of this solution will you need to supply the needed 0.5% of the patient? A. 1.0 g В. 1.5 g С. 2.0 g D. 4.0 g 2. Given the amount calculated on the previous item, and if the E value of phenylephrine HCl is 0.32, calculate its corresponding sodium chloride tonicic equivalent. А. 0.16 B. 0.64 С. 1.60 D. 6.40 3. If the E value of nafazolin is 0.27, what is the sodium chloride tonicic equivalent of the patient's needed nafazolin? A. 0.0135 B. 0.1350 1.3500 D. 13.500 4. What is the combined sodium chloride tonicic equivalent of the remaining ingredients (edetate disodium (E =0.24) and sodium metabisulfite (E = 0.7)? A. 1.060 B. 0.106 С. 1.290 D. 0.129 5. To make the solution isotonic, what…A.How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?After blood collection, the red cells are separated from the serum to be used for the preparation of the stock solution. How is it done? For the serial dilution, your stock solution must have a concentration of 3.5 mg/mL. How much diluent must be added to the 5.3 mg/mL red cell to prepare the stock solution? Show pertinent solution/s. What is the appropriate diluent used for the preparation of the red cell suspension?
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