Which statement is correct about expression of a gene regulated by Gal4? O Galactose increases gene expression by inhibiting Gal80 O Galactose increases gene expression by binding Gal4 O Galactose decreases gene expression by binding RNA polymerase O Gal4 binds to the promoter Gal80 binds to the promoter
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- What is the role of general transcription factors? GTFs bind to enhancers or silencers and regulate transcription GTFs bind to the core promoter and allow transcriptional initiation GTFs are cis-acting regulatory sequences GTFs regulate the length of the mRNA GTFs are part of the RNA polymerase II holoenzyme, and control transcription initiationThe following diagram depicts the elements at the: Ac TTC g99 Ea cgcc tata an! ATT BRE TATA box Inr DPE (TBP) TFIIB TEIID Promoter Enhancer O transcribed gene O SilencerIn the regulatory switch experiment, which level of gene expression regulation is the focus? Regulatory switch Enhancer Mouse Enhancer Bat Enhancer Bat-mouse translation level regulation transcription level regulation post-translational regulation O post-transcriptional regulation (RNA modification)
- Cancers are often caused by overactive growth factor receptor signaling (remember growth factor receptors are enzyme-linked receptor pathways). If you were able to use gene therapy to overexpress a particular protein in a cancer cell, which of the following might be useful to overexpress in order to combatt cancer? a GAP that acts on Ras a phosphatase that acts on GPCR a protein that enhances the activity of Akt a ubiqtuin ligase that acts on the MAPK phophatasee table below with the word that matches the definition in the right column. regulatory proteins (transcription factors) that speed up transcription by helping RNA polymerase bind to a promoter DNA sequences that indicate where transcription of a gene by RNA polymerase begins-typically located upstream from the 5C end Proteins bound to DNA that affect when and how fast a gene is transcribed These factors slow down/shut off transcription either by preventing access to promoters or by keeping RNA polymerase from moving along the DNA strand 3 Regulatory DNA sequénces located upstream or downstream from an associated gene that enhance the transcription rate of that gene when bound to specific proteins 3A sequence of DNA that when bound to a transcription factor blocks the inappropriate effect of an enhancer on a neighboring geneLack of phosporylation of the C-terminal domian of Pol Il will result in which of the following? O Assembly of the full set of general transcription factors plus Pol II at the promoter, but unphosphorylated Pol II cannot leave the promoter region No assembly of the general transcription factors at a promoter No binding of Pol II at a promoter The unphosphorylated Pol II can initiate and elongate but cannot terminate
- Match each example of mutation to the correct effect on function. Hypomorphic mutation Hypermorphic mutation Antimorphic mutation Neomorphic A deletion results in the loss of part of a protein and the protein retains some of its normal activity. A mutant allele makes a protein with increased cataytic activity. A mutant form of a transcription factor binds new DNA sequences to activate different genes. A mutant from of a receptor protein interferes with the function of the wild-type receptor through heterodimerization.Which of the following functions are characteristic of general transcription factors in eukaryotes? Hint: there may be more than one correct response. Be sure to check all that apply. They inhibit RNA polymerase binding to the promoter and begin transcribing. They bind to other proteins or to the TATA box. They are sufficient to allow high levels of transcription. O They initiate and regulate the transcription of genes. O They bind to sequences just after the start site of transcription.LysR regulators like llvY repress transcription from their own promoters. This is called a negative feedback loop. Why might negative feedback be a good way to control expression of a transcriptional repressor? This type of negative feedback loop ensures that ilvC transcription will always be repressed when llvY levels are low, and ilvC will be transcribed highly when llvY levels are high. This type of negative feedback regulation ensures that ilvY is never transcribed. Since llvY is a transcriptional repressor, it will slow down cell growth by blocking transcription. So, llvY represses it's own transcription so that no llvY can ever be made, and the cell can grow. This type of negative feedback loop ensures that there is always a moderate amount of llvY. When IlvY levels fall too low, transcription from the promoter will increase since IlvY won't be able to repress it's own promoter. This negative feedback also ensures that IlVY levels don't rise too high - when llvY levels are high,…
- Gene expression may change in response to external cues through the activity of multiple signal transduction cascades. What is the primary mechanism that determines if a gene is expressed or not?Modify the diagrams below to reflect the activation and repression of lac Use arrows, lines, X’s, etc. to illustrate control of gene expression.which of the following correctly describes how protein kinase A can activate genes? A: nuclear protein kinase A is activated by cAMP to phosphorylate general transcription factors B: cytosolic protein kinase A is activated by cAMP to release the catalytic subunits, which move into the nucleus and phosphorylate CREB C: cytosolic protein kinase A is activated by cAMP to release the catalytic subunits, which move into the nucleus and phosphorylate general transcription factors D: G protein-coupled receptors may be desensitized by serine phosphorylation