9Which of the following is/are true regarding SDS-PAGE (there may be more than one correctanswer, select all that apply)?Proteins are separated based on size and charge.SDS is used to coat the proteins in a positive chargeProteins are separated based only on charge.Proteins are separated based only on size.The smallest proteins travel the farthest

SDS-PAGE is the abbreviation for Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis.It is a…

Answered: Which of the following is/are true…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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On an SDS-gel, If the distance traveled by the bromophenol blue dye is 7 cm, and the distance traveled by the protein band is 2.8 cm, the mobility of the protein is 40 4 40% 0.4
A C2 G This a photograph of an actual polyacrylamide gel that has been run with samples of protein. For each question, write the letter from the item in the gel that best matches the word or concept that is described. Answers may be used once, more than once, or not at all. There may be more than one correct answer per question. 1. Band 2. Lane 3. Well 4. Will protein migrate through the gel from A to B or will it migrate from B to A? 5. Which end is nearest the negative electrode, A or B? 6. Which contains smaller protein fragments, F or G? 7. Which letter indicates the highest concentration of protein fragments that are the same size 8. Where would a sample of protein be loaded? B.
Under what pH conditions can a protein not bind to the beads in a column? pH = -pKa pH = pI pH = 7 pH = pKa In size exclusion/gel filtration chromatography, the elution order is dependent upon Molecular weight Concentration Overall Charge Enzymatic Activity
Mass spectrometry and X-ray diffraction are common biochemical techniques for characterizing proteins. Classify each statement based on whether it applies to mass spectrometry, X-ray diffraction, or both techniques. Mass spectrometry protein must be conformationally stable X-ray diffraction small amounts of protein are sufficient analysis requires information about the protein-coding gene or genes Answer Bank protein may be denatured requires a large amount of protein protein sample must be pure can detect a covalent modification to a protein Both techniques
Under what pH conditions can a protein not bind to the beads in a column? pH = 7 pH = pI pH = pKa pH = -pKa
In SDS-PAGE, which of the following are true? Select all that apply The protein samples are separated based on their size The protein samples are denatured by SDS and heat The overall charge of a protein is negative The overall charge of a protein is positive
By increasing the percentage of acrylamide in an SDS-PAGE gel, one will observe the following change in the range of molecular weights of the observed proteins. Decreased range of molecular weights observed. No change in range of molecular weights observed. Increased range of molecular weights observed
If you crate the absorbance spectra of a solution containing dna protein and buffer using buffer as a blank the resulting spectrum should look like protein alone buffer alone protein and buffer protein and dna
a 2D gel electrophoresis was done on the proteins found in the chart. create a chart with the correct parameters on both dimensions using 1-6 to indicate the position of each protein on teh 2D gel.
Gradient PAGE uses differing gel concentrations along the length of the gel to achieve optimalseparation of proteins of a wide range of molecular weight proteins. How does the staining pattern of these types of gels differ from nongradient PAGE?
Choose the FALSE statement about protein electrophoresis. The proteins in the gel can be visualized by staining them with silver. The distance and speed that a protein moves depend on its shape. Small proteins move rapidly through the gel. It is performed in a thin, horizontal slab of polyacrylamide. The gel serves as a molecular sieve that enhances separation.
Which of the following is NOT a unit of concentration? All of these options are units of concentration. mM Moles/Liter Molar You gathered your data from Lab , and found the following values: Total Activity of lysozyme in HEW: 14 units Total Activity of lysozyme in carb 1: 21 units Total amount of protein in HEW: 70 mg Total amount of protein in Carb 1: 15 mg Calculate the extent of purification of lysozyme in Carb 1. A 7 fold B 150% C 15 fold D 70%

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