What order should the steps be in for this culture method?

Q: what is the procedure and materials you need to stain Staphylococcus aureus?

A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium hat belongs to the Firmicutes and…

Q: What is the function of the mordant used in the Gram staining procedure? If the iodine step is…

A: Gram stain or Gram staining, likewise called Gram's strategy, is a technique for staining used to…

Q: How does incubating plates at 37°C influence the results of the quantitative dilution and plating of…

A: Under the regulated conditions of the laboratory when microbial cells are allowed to grow and…

Q: What is the purpose of flame-sterilizing the mouth of the test tube and the side of the Petri dish…

A: Flame is an excellent way of cleaning a surface from contaminating organisms. Instant high heat…

Q: Describe how the streak-plate method is used to obtain a pure culture and how the resulting culture…

A: In microbiology, culture refers to microbial growth of a certain species maintained in controlled…

Q: Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?

A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…

Q: Why each bacterium must be tested separately?

A: Antibiotics are chemical substances that kill or inhibit or prevent the growth of microbes. They are…

Q: Which microbe requires serum components to be added to the growth medium?

A: Some microbes are nutritionally fastidious and hence needs some substances to be added to the growth…

Q: In this experiment, a culture was serially diluted to the concentrations below. Each plate was…

A: A "microbe" is a living entity that is so tiny that it cannot be seen with the naked eye.…

Q: What are the 4 types of culture?

A: Biological cultures include common laboratory means, which are involved in studying living organisms…

Q: How can the enrichment culture technique be used medically?

A: Bacteria require appropriate environmental conditions for its growth. Solid or a liquid preparation…

Q: Why is the loop flamed before it is placed in a culture tube? Why is it flamed after completing the…

A: The term sterilization refers to any process that eliminates, kills, removes, or deactivates all…

Q: With a plate count average of 297 and a final dilution on the plate of 1:1,000 ; what is the viable…

A: Bacteria are the prokaryotic, unicellular organisms that are sometimes pathogenic in nature. The…

Q: What is the application of differential centrifugation?

A: In the laboratories, different kinds of techniques and devices are used to separate components of a…

Q: describe the agar-overlay technique

A: Biological techniques are processes or methods for trying to study living things. They include…

Q: What should be taken into consideration in the selection of the culture preservation method?

A: The problem of maintaining microbial culture is one that many fields of microbiology face. The…

Q: What are the equipments/ materials used in imoobilizing and separating cells when using: spread…

A: Aseptic techniques refer to any method which is used to sterilize and maintain sterility of the work…

Q: What is the difference between streak plate method and spread plate technique?

A: Answer of the question given below...

Q: Is the Peptone Iron Agar (PIA) a complex or defined medium? Explain based on its composition. Is the…

A: Depending on their composition, media can be classified as defined and undefined media. Defined…

Q: If SIM medium was used for motility determination for Proteus vulgaris, what noticeable change to…

A: What is motility? Motility is the ability of an organism to move by itself. Eucaryotic cells use…

Q: What is the most important instrument in an animal cell culture laboratory?

A: The process by which animal, insect or human cells are isolated from a living tissue and grown in…

Q: what is the culture media for gram-positive bacteria?

A: The culture media for gram positive bacteria is Mannitol salt agar . The presence of bile salts, as…

Q: Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?

A: Microbial taxonomy is a field of biological sciences that deals with the grouping of microorganisms…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: The statement, “In the laboratory, a sterile inoculating loop is moved across the agar surface in a…

A: The technique is streak plate technique. Streak plate method is a pure culture technique which helps…

Q: When using the blue/ white colony screening method, what do blue colonies represent ?

A: Blue- white screening is used for the identification of recombinant bacteria.

Q: What is selective medium?

A: Description of a selective medium.

Q: What are the advantages and disadvantages of using the Wet Mount technique?

A: As per bartleby guidelines, we are suppose to answer only one question kindly post other separately.…

Q: why doe the incubation time of harada mori method requires up to 10 days?

A: Introduction:- Humans are usually infected with S. stercoralis by infective filariform larvae…

Q: What should be considered when preparing a monolayer cell culture?

A: Monolayer culture, also known as adherent culture, is a form of animal cell culture in which cells…

Q: If you count 70 colonies from a urine culture obtained using 0.001ml calibrated loop, how many…

A: CFU or colony forming unit is an unit of measurement that is used to estimate the viable microbial…

Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…

A: Endospore stain is a differential stain that is used to image endospores in bacteria. A few genera…

Q: How does the agar dilution method differ from streaking toobtain isolated colonies?

A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…

Q: Describe conventional techniques used to isolate single colonies and establish a pure culture

A: Answer: Introduction: Pure culture a culture contains growth of only single organism without other…

Q: What things can be done during sample collection of a sick patient to help eliminate the culture of…

A: Normal flora is the organisms that are present in the body for normal functioning. The normal flora…

Q: Explain why culture based biochemical and genetic testing would not be the ideal method to identify…

A: Microbial culture or microbiological culture is a technique in microbiology in which microorganisms…

Q: How do we reconcile the number of colonies in the plates to the actual number of bacteria in the…

A: Microbes may enter the body in a variety of ways and cause infection everywhere, but antigen and…

Q: what is the procedure of solving the probelems of cultures those are suffering severely due to…

A: This is the defense compound of the plant and are toxic in nature because of that preventing plant…

Q: What microbe requires serum components to be added to the growth medium?

A: Introduction Microorganisms:- An organism that can be seen only through a microscope, They live in…

Q: What advantage does the oil immersion objective have over the low power objective when doing…

A: Introduction:- Bacteria are prokaryotic and very small sized organism's . They can not be visualised…

Q: What are the different manufacturing methods of Suppositories dosage form? Explain heat molding…

A: Suppositories are the solid medication  that delivers drug in rectum ,vagina and or urethera. Most…

Q: Which culturing method provided the clearest results, the semisolid medium tube or the soft agar…

A: Agar is a jelly-like substance obtained from red algae. It is a mixture of two components namely…

Q: In the entrropluri tube system which test require the addition of reagents after the incubation time…

A: Entreropluri test system is a Enterobacteriaceae identifying system. It is use to identify some gram…

Q: Why are aseptic techniques implemented during inoculation?

A: Aseptic means without contamination and Inoculation means transfer of a bacterial sample onto a…

Q: What is meant by the word sterile? Why is aseptic techniquenecessary for successful maintenance of…

A: Microbes are all pervading. They are found everywhere, in air, water, any substratum, inside and on…

Q: What staining method would you use to verify that the isolate is not producing survival structures…

A: Staining is the procedure that helps to visualize the minute organisms under a microscope using…

Q: What is the purpose of using the streak plate method?

A: Solution-  Introduction -  Streak plate technique is used  for the isolation into a pure culture of…

Q: What type of reaction or lab test differentiates the presence of either staphylococcus bacteria or…

A: Staphylococcus Bacteria which grows in cluster are Gram-positive (catalase-positive). Streptococcus…

Microbial Growth

The development of microbial growth is influenced by various natural conditions (both physical and synthetic) like pH, temperature, saltiness, gas focus (O2, CO2, and H2), nutrients, water action, and assimilation. Knowing the distinctive physical and chemical factors that influence the development and endurance of microbial growth in the climate will assist with reinforcing endeavors outfitted towards their control. These lines lessen or diminish their harmful impacts on humankind, creatures, and the climate.