What method is best for for isolating a colony on a TSA plate? A. Swab plate B. Straight line inoculation with a loop C. Streak plate with a loop D. Spread plate with a glass spreader
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What method is best for for isolating a colony on a TSA plate?
A. Swab plate
B. Straight line inoculation with a loop
C. Streak plate with a loop
D. Spread plate with a glass spreader
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- While conducting the Hydrogen Sulfide Test (SIM Agar) you open up one of the tubes and it gives off a rotten egg smell while another tube you cultured does not. What does the smell indicate? How come there was no change in color in the tube that indicated a positive test? Please explain how the color change is to occur. Think critically.Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observedWhat is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handle
- What are the basic differences between the three plating methods? Fill up the following table. Streak Pour Spread Equipment/ Materials used in immobilizing and separating cells Purpose(s) (isolation, enumeration, both) Type of colonies (surface, subsurface, both) Advantage DisadvantageWrite the letter of your choice at the start of the sentence then support it with a short explanation. Plaque assay and Kirby Bauer assay are similar in terms of A. they both use a bacterial lawn B. zone of inhibition is measured C. antibiotics are used D. agar overlay is added on solidified agarA capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C Simple Stain D Differential stain E Negative Stain
- Describe the process to make a 4-phase streak plate beginning with your first streak (i.e you have bacterial culture on your sterile loop and are about to start to streak your agar plate ).In product testing, no colonies appeared. Interpretation: Rationale:Which of the following would suggest your stock culture is contaminated? Which of the following would suggest your stock culture is contaminated? Gram stain indicates Gram-positive bacilli. Broth media become turbid. Gram stain indicates both Gram-positive cocci and Gram-negative bacilli. Colony morphology is uniform on the streak isolation plate. Gram stain indicates Gram-positive staphylococci.
- In spread plate method, colonies form within the agar and agar surface. In pour plate method, previously prepared agar plates are not required. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For quadrant streaking, the loop must be flame sterilized between quadrant streaks. a. First statement is TRUE, Second statement is FALSE. b. First statement is FALSE, Second statement is TRUE. c. Both statements are TRUE. d. Both statements are FALSE.For Staphylococcus aureus, indicate which tests need to be done & what media you need. Fill in box provided Test Media Needed