What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantage of heat fixing?
Q: What is the usual stain used for staining a sputum smear?
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Q: What are the principle and basic concepts of DIFFERENTIAL staining?
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Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…
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Q: Microbiology
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Q: What will happen if you heat fix ypur smear for flagella staining?
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Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
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Q: I Why does't a negative stain colorize the cells in the smear?
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Q: What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant?
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Q: 1.)What is the purpose of a counterstain?
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Q: What are the principle and basic concepts of Simple staining
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Q: What is the usual stain used for staining a sputum smear?
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Q: 1-What microbial characteristics can one ascertain from a simplest stain? 2-A student is directed…
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Q: 1. What is one advantage of utilizing the pour plate technique over the streak plate technique ?
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1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain?
2. Why is it necessary to make a heat-fix smear and what are the disadvantage of heat fixing?
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- 1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantages of heat fixing? 3. what is the best age for your culture when performing a gram stain? Why? 4. Why is Gean staining considered a differential staining process? 5. What are some reasons a Gram positive cell might appear Gram negative?1-What microbial characteristics can one ascertain from a simplest stain? 2-A student is directed to make a simple stain of coli with crystal violet, but got mixed up and used safranin instead. How would their observations be different? Would the information obtained be any different? 3-How is the procedure different when taking cells from a solid medium compared to taking cells from a liquid medium? Why is it important that your smear be thin?1. Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?2. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method. 3. What is the specific diagnostic value of this staining procedure?4. What is the specific diagnostic value of this staining procedure?5. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method.6. What is the specific diagnostic value of acid fast staining procedure?
- 1. What is the significance of soaking the container in bleach? 2. What is the significance of keping the container closed except when pouring the medium; partially opening the container during pour-plating in preparing culture media?1. What is the usual stain used for staining a sputum smear? 2. Give the principle of acid fast staining and gram staining.1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?
- 1.why is gram stain considered a differential stain? 2.How do gram positive and gram negative bacteria differ in cellular structure, and how does this contribute to their differential staining properties? 3.How does the age of a culture affect the gram stain reaction? What is an optimum culture age for a valid gram reaction? 4.Which step in the gram stain procedure is most prone to error?If done correctly how might that step affect the end result? 5.what is the function of mordant, and which reagent serves this purpose in the gram stain procedure? 6.List the reagents of the gram star technique in order and their general role in the staining process. 7.In what type of cell, gram -positive or gram-negative , would you find lipopolysaccharide in its cell wall?2. Give the purpose of each of the following reagents in a differential staining procedure: (Primary stain, Counter stain, Decolorizing agent and Mordant)4. What are the three parts of a stain? 5. Why is it critical to allow cells to air dry before heat fixation? 6. Give an example of a basic stain and an acidic stain.
- 4. Why is it necessary to let bacterial smears completely air dry before heat fixing?1. Define Differential Staining. 2. Application of differential staining technique.1. What are the advantages of staining a bacterial preparation before observing it under a microscope?2.Briefly state how a hanging-drop preparation is prepared.3. How does the heaviness of a bacterial smear affect its microscopic analysis4. Why should you be careful not to underheat a smear during the heat-fixing process5.What is heat fixation? How is it carried out?6. Why do you think the presence of grease or dirt on a glass slide will result in a poor smear preparation? Cite two or three reasons 7. Why are basic dyes more effective for bacterial staining than acidic dyes?8. State two ways that can confirm whether a bacterial smear has been correctly prepared or not