Q: What is the purpose of the alcohol (OH) in the gram staining process?
A: Usually, microbes are colorless, so it’s very difficult to see them even after magnifying under…
Q: hello, can you draw the following on endospore staining in 1000x objective? thank you. - 1 day…
A: Bacillus is a genus which is a endospore forming, rod shaped, gram positive bacteria.
Q: What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate,…
A: PURPOSE OF POUR PLATE TECHNIQUE The pour plate techniques can be used to determine the number of…
Q: What are the sources of error in performing Gram staining?
A: Bacteria can be divided into two categories namely gram positive(with thin layer of peptidoglycan)…
Q: What is gram staining and its purpose? Why is it only applicable in bacteria?
A: Introduction: Differential staining methods like Gram staining, acid-fast staining, endospore…
Q: In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside…
A: Several laboratory techniques necessitate smear preparation. A smear can be made using either broth…
Q: Why is the Gram stain considered a differential stain?
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: Describe the bacteria observed in different staining procedures. Please refer to the photo -…
A: Bacteria can be stained by a variety of stains so that they can be visualised in contrast to the the…
Q: shape
A: There are different shapes of bacteria and different classes of bacteria on its shape These shapes…
Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…
A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…
Q: I need to discribe the "the colors of gram+ and gram (-) bacteria" in each independent step of the…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: If you were working with an unlabeled simple stained smear, would you be able to identify the…
A: Staining is used to colour biological specimen such as cell to identify them. Simple staining…
Q: Why is it necessary to use a 24-hrold culture in gram staining?
A: Note: Since you have posted multiple independent questions in the same request, we will solve the…
Q: Which of the following serves as a counterstain for the gram-stain technique? It also happens to be…
A: Gram staining is a technique used to differentiate two groups of bacteria based on the different…
Q: citrobacter freundii
A: Citrobacter freundii is a species of anaerobic gram-negative bacteria which belongs to the family…
Q: Why should a young culture be used in gram staining rather than an old culture? b. Why is it…
A: To answer this question first, we need to understand what is gram staining and what are the steps of…
Q: Describe one error in the staining procedure that would result in a Gram-positive bacterium…
A: Gram staining is a differential staining technique that differentiates between a gram-positive and a…
Q: why is it necessary to employ a spore stain rather than rely on diagnosis of sporing from…
A: Spores are cellular structures produced by plants, bacteria, and fungi. Spores can be produced for a…
Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…
A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…
Q: two different
A: Gram staining- A test that checks for bacteria at the site of a suspected infection such as the…
Q: list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet…
A: Endospores are structures produced within certain bacterial cells that allow them to survive harsh…
Q: Based from the results obtained from Gram staining, explain why bacterial cells retained or changed…
A: Staining is a technique that is used to enhance the contrast of the microscopic image through the…
Q: How would you expect the staining properties of 24-hour culture of Bacillus subtilis or the other…
A: Bacillus subtilis is a very well bacteria that is widely used as a Gram-positive bacteria model…
Q: The decolorizer used in the Gram Stain, and the decolorizer used in the Acid Fast Stain, are the…
A: Hi! Thank you for the questions. As you have posted multiple questions, I will be answering the…
Q: The Gram staining procedure involves multiple steps with use different reagents. Complete the table…
A: Step 1 Christian Gram in 1884 developed a technique of staining bacteria called Gram staining.…
Q: Staining bacterial capsule can not be accomplished by?
A: Since most standard stains cannot enter the capsule because it is so tightly packed, it is difficult…
Q: What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic…
A: Endospore stain is a differential stain that is used to image endospores in bacteria. A few genera…
Q: Why is it called as differential stain
A: Staining is a technique used in slide preparation to color the specimen.With light microscopy,…
Q: if there are features of the gram stain that are not as expected what possible sources or error may…
A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…
Q: Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct…
A: The cell wall of most bacteria has an overall net negative charge and thus can be stained directly…
Q: what is the function of each one of the Gram-stain reagents?
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: What is the function of a mordant, and which reagent serves this purpose in the Gram stain…
A: An extensively used microbiological method of staining is called a Gram stain. It significantly…
Q: How does the age of a culture affect the Gram stain reaction? What is an optimum culture age for a…
A: To identify the unknown bacteria this gram staining is used. This staining technique differentiate…
Q: What is the theory about the mechanism of the Gram-stain reaction? please include…
A: The mechanism of gram staining is employed essentially to differentiate between gram-positive and…
Q: What is the advantage of gram staining over simple staining
A:
Q: What is the purpose of iodine in the gram staining process? Explain.
A: Gram stains are used to distinguish between bacteria with and without cell walls. When the gramme…
Q: Why can't gram staining be used for mycobacterium?
A: Gram staining is one of the staining techniques. This is usually used for differentiating between…
Q: Since the dyes for the Gram stain are not specific to the bacteria theystain, what mistakes in the…
A: Gram staining is a technique used for staining the microbial samples. It was performed by the…
Q: the chemicals used in the gram staining method?
A: The bacteriologist Hans Christian Gram was developed gram staining method in 1884. the Gram staining…
Q: what are the reagents for the four major steps of the gram stain procedure
A: Gram staining protocol is used to differentiate between Gram-positive and Gram-negative bacteria by…
Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…
A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…
Q: What is acid fast staining and why do we use gram staining more?
A: Staining is a method in which images of specimens enhanced at microscopic level. It is commonly used…
Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…
A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.
Q: Endospores can be seen as refractile structures in unstained cells and as colorless areas in…
A: Endospores are dormant, non-reproductive structures formed by certain bacteria during unfavorable…
Q: What are the principle and basic concepts of GRAM STAIN?
A: Gram staining is among the most important microbiology staining procedures. It was first invented in…
Q: In preparing bacterial smear, why do we need to pass the slide over the flame?
A: since this is a multi-part question, so I am answering only 1st part. please upload other questions…
Q: Does the gram staining method still have a place in today's world of advanced diagnostic methods? If…
A: Gram staining is a type of staining which is used to differentiate bacteria on the basis of…
Q: What staining technique is used here? What is the primary stain, decolorizing agent, and counter…
A: Gram Staining is the common, important, and most used differential staining techniques in…
Q: To dilute a bacterial culture, 500 μl of a 16 hour culture is mixed with fresh culture media to a…
A: Given Values: Volume of the 16 hour culture = 500 μl Final Volume of the culture media = 5 ml
Both the Endospore Stain Protocol and Mannitol Salt Agar Media are differential. How can this be so?
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- Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?Why is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet only?.Why does a streaking method used to inoculate plates result in isolated colonies? (Explain systematically)
- Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayWhy is the looping out method preferable for sputum specimen for acid fast smears?list the major steps, in sequence needed to perform the schaeffer-fulton and crystal violet endospore staining methods. (omit the rinsing steps)
- Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain procedure? Why or why not?Why does a streaking method used to inoculate plates result in isolated colonies?What would be the expected outcome of an endospore stain test If the bacteria is a pathogenic intestinal bacteria?