What is the main advantage of fluorescence polarization? Sensitivity of fluorescent probes Low cost of instrument and reagents Compatibility of reagents with most analytes No need to purify samples Easy to attach fluorescent probe
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- How could you utilize fluorescence quenching in the different analytical application? Please answer shorty at your own words. Answer should be to the point.What can be the errors and limitations of Gel Electrophoresis practical? Explain in very much detail and also provide some better suggestions.How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: O Water must be added to a smear from liquid media but not from solid O Water must be added to a smear made from solid media but not liquid media A smear made from liquid media will be more thick than a smear made from solid O A smear made from liquid media will be thinner than a smear made from solid media O A smear made from liquid media doesn't have to be fixed but a smear from solid media does Microsoft Bing 11:31 AM 87°F Sunny 9/14/2021
- Which of the following statements is FALSE? Question options: stains increase the contrast of a specimen with its surroundings all of these statements are TRUE dyes generally consist of a chromophore and an ion the chromophore of an acidic dye is positively-charged simple staining procedures use a single dye basic dyes bind to negatively-charged moleculesPut the following steps for running a gel in order. first Remove comb and put gel int v [ Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel second Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn vWhy is UV light used in Column chromatography experiment ?
- Discuss the functions of the following components in performing gel electrophoresis; Polyacrylamide gel Power supply TBE buffer solution Restriction enzymesWhat are the limitations of UV Visible spectroscopy and fluorescence spectroscopy? Include reference link if possibleExplain the function of stacking gel, separating gel, and Coomassie blue stain in the electrophoresis experiment.
- How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: A smear made from liquid media will be thinner than a smear made from solid media A smear made from solid media doesn't have to be fixed but a smear from liquid media does A smear made from liquid media will be more thick than a smear made from solid Water must be added to a smear made from solid media but not liquid media A smear made from liquid media doesn't have to be fixed but a smear from solid media does Water must be added to a smear from liquid media but not from solidNative gel electrophoresis56/take Select all that apply to a direct simple stain: O uses only 1 dye in the procedure O uses multiple dyes in the procedure O can be used to determine cell morphology, size, and arrangement. does not involve fixing does involve fixing does not involve a washing step does involve a washing step O the dye is basic or positively charged and stains the bacteria O the dye is acidic or negatively charged and stains the background O cells may be distorted or shrunken Ocells will never be distorted or shrunken