What is the direction of transcription in the plasmid shown below? Explain your answer based on the features present in the figure.
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What is the direction of transcription in the plasmid shown below? Explain your answer based on the features present in the figure.
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- Apol (5322) Apal (5258) lacl ApoI (4612) tac promoter pGEX-6P-1-INIA 6347 bp PstI (3301) Ampicillin GST C3 cleavage site Apal (941) Bam HI (946) Pst I (1091) Pst I (1412) inlA funct dom Pst I (1937) NotI (2339)In your own words, explain the term transposon tagging.Consider the following simple regulatory pathways. Assume the full pathway is shown. A- E- B- F- C- G- D- 1 H- A You identify several null mutations (a complete deletion of the gene). For each mutant (ind with a - sign), determine whether the final product (I, J, K or L) is inducible, uninducible, or constitutive. 2 B 3 C 4 D inducible inducible constitutive uninducible constitutive inducible inducible E uninducible F G H > > >
- The sigma-70 promoter is very well-studied. The Sigma-54 promoter, on the other hand, hasn't had quite as much study devoted to it, but a consensus sequence for Sigma-54 promoters has been determined, and it looks like this: C GAAA FICGCACTECT УА C 5-26-25-24 -23 -22 -21 -20 -19 -18 -17 -16-15-14-13-12-11 -10 3 wablogo berkalay.edu (From Franke et. Al, 2011 BMC Genomics) If you were studying a gene with a promoter that exactly matched the consensus sequence, which base would, if mutated, probably make the largest difference in the ability of Sigma-54 to bind to a promoter? O Base position 26 Base position 10 Base position 12 Base position 16draw the p21 promoter. Your drawing should include (1) the start site, (2) the TATA box and (3) the ERE/AP-1 binding site5 5 S 6 5 5 5 6 U 6 U 6 5:14 PM | 0.2KB/s HHHHH R R U RUUR ARU AP AP R U U R R AP R R R AP MOLECULAR...GENETICS. Describe gene regulation at transcription level. Explain the role of antsense RNA in control mechanism. Describe translational control mechanisms. Describe common DNA damages. Distinguish excision and mismatch repair. Describe the role of recA protein in recombination repair Elaborate on SOS repair mechanism. Define thymine dimer. How are they formed and repaired? Describe the molecular basis of mutation. 11 Leu+ Met+ Arg+ Write a detailed note on spontaneous mutation. Explain about mutant detection methods. Define reverse mutation. Describe the mechanism underlying Intragenic and intergenic suppressor mutations Describe the transposition mechanisms. 13 Vo LTE UNIT IV Time (Min) Describe the process of generalised transformation occurring in bacterial chromosome and plasmid. Elaborate on molecular mechanism and significance of transformation 22 Describe the process of…
- You are attempting to prepare a single gene knockout library using the pRL27 transposon system. You grow the donor E. coli in Luria broth containing both kanamycin and diaminopimelic acid (DAP) and your recipient Serratia rubidaea in plain Luria broth. You combine an equal ratio of donor and recipient cultures and plate the mixture onto Luria agar supplemented with DAP. After 24 hours incubation at 37°C, you create a cell slurry and plate the cells onto Luria agar aupplemented with kanamycin. After 24 hours incubation at 37°C, you find that no colonies grow. What best explains this outcome? A. Failure to supplement media with DAP B. Failure to remove antiobiotic containing media C. Failure to incubate for a sufficient length of time D. Failure to incubate at the appropiate temperature E. Failure to use the proper mating mix ratioAuxotrophic mutation 103 grows on minimal medium supplemented with A, B, or C; mutation 106 grows on medium supplemented with A or C, but not B; and mutation 102 grows only on medium supplemented with C. What is the order of A, B, and C in a biochemical pathway?Consider the gal10D56 reporter gene. In 300 words or fewer, describe 1) the role of GAL7 in galactose metabolism and its importance for cell function 2) the mutation present in the gal10D56 reporter gene 3) the consequence of this mutation for GAL7 expression in wild type cells, 4) the mechanism by which certain mutations can suppress the effects of gal10D56, and 5) the specific purpose for using this reporter gene.
- Are double-knockout animals (DKOs) and even triple-knockoutanimals (TKOs) also possible ?A number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the highest beta-galactosidase enzyme activity, when grown in the lactose medium. O CAP+ r* p* o* z O CAP* I P* o* z* O CAP* r* P O* z* O CAP I P* O z*Strain ROFL4 has a premature stop mutation in the lacZ gene, resulting in a nonfunctional b-galactosidase. Otherwise all other parts of the operon are functional. Circle the least number of components for an F' plasmid that will restore normal regulation and function of the lac operon in the resulting partial diploid. (may need more than one) (a) lacI+ (b) lacO+ (c) lacP+ (d) lacZ+ (e) None, cannot be restored.