What can be interpreted when the Benedict's test is applied to enzymatic hydrolysis of sucrose (yeast- hydrolyzed sucrose)?
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What can be interpreted when the Benedict's test is applied to enzymatic hydrolysis of sucrose (yeast- hydrolyzed sucrose)?
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- There is a cysteine protease, which uses a similar chemical mechanism to chymotrypsin except for using cysteine instead of serine during catalysis. Please draw the stepwise chemical mechanism for the cysteine protease.Why do we use the artificial substrate p nitrophenyl glucopyranoside instead of the natural substrate cellobiose to determine enzymatic activity of Cellobiase?whyThe method of oxidation of galactose by concentrated nitric acid is used to detect this sugar?
- Why is maltose reduced to Benedict's solution and fermented by yeast?What is the optimal temperature for the enzyme shown above?What is the committed step of pyrimidine biosynthesis? Include the names and structures of any product(s), substrate(s), and cofactors, and the name of the enzyme responsible.
- The mechanism involved in the reaction catalyzed by phosphoglyceromutase is known to involve a phosphorylatedenzyme intermediate. If 3-phosphoglycerate is radioactively labeledwith 32P, the product of the reaction, 2-phosphoglycerate, does nothave any radioactive label. Design a mechanism to explain these facts.Why is gluconeogenesis labeled as enzymatic by-pass reactions?UDP-glucuronosyltransferase enzymes bind the organic compound UDP-glucuronic acid (UDP-GA) in order to catalyse the transfer of a glucuronic acid group from UDP-GA to a drug molecule, releasing UDP from the active site as a product. UDP is then regenerated by the activity of another enzyme. What terms could be used to describe UDP-GA?
- What is the enzyme classification of the catalyst used in the conversionof oxalosuccinate to alpha-ketoglutarate?Name the substrate for each of the following enzymes and the products produced from the enzyme-catalyzed reaction: amylase, protease, peptidase, and lipasea) Write the catalytic mechanistic steps used in the urea cycle. If transamination is required, showonly the net reaction of this step.b) Where do the 2 nitrogen atoms and the 1 carbon atom that make the final product come from?