What are the biochemical test used to assay fatty acids and cholesterol?
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- milk digestion and meat digestion tests are biochemical tests?Discuss the principle behind Salkowski test, reaction with ammonium ferrothiocyanate, and saponification to differentiate TAGs, phospholipid, and cholesterol. What are the general reactions involved? Include chemical reactions if necessary.What is reducing sugar? What tests can be used for the qualitative determination of reducing sugars?
- What are reducing sugars? Why are monosaccharides considered as reducing sugars? Which qualitative tests can help you determine the presence of these reducing sugars and the principle behind it?What is the principle involved in the method used in determining blood glucose? Explainhow does ninhydrin detect amino acid?
- Serum blood of a patient with dislipoproteinemia type 1 has milky appearance even in fasting. If serum stays at low temperature (40) for several hours fatty layer appears on its surface. What are the possible causes of these symptoms? To explain this, answer the questions and do the following tasks: a) what compounds of serum must be tested for that patient in biochemical lab? b) write the reaction which does not occur properly in patient’s blood; c) write down the schemes, explaining how the products of the previous reaction are used in adipose tissue and heart in healthy person 2 hours after a meal.Which of the following is incorrect about blood glucose test interpretation A) fasting plasma glucose 6.1-6.8 defined as having IGT and should probably be screened again after a year or so. B) 2 results>7mmmol/l defined as having diabetes C) fasting plasma glucose >6mmol, recall for further test. D) fasting or random plasma glucose, on one reading of <11.1mmol/l is diagnostic of diabetesDescribe various detection methods used in High-Performance Liquid Chromatography (HPLC) and types of biomolecules that are detected in each method?