Use the blanks on the left hand side to put the following statements in order (use numbers or letters to designate the order). All of the statements refer to the diauxic (sequential) growth of a bacterium on the carbon substrates glucose and lactose (see textbook Fig 4.11 on diauxic growth). Remember, the lac operon contains the genes necessary for the lactose catabolism. The glucose substrate is completely consumed, at which time the repressor protein on the lac operon unbinds from the DNA molecule. The lactose substrate is catabolized by the active B-galactosidase enzyme. Glucose, as the preferred carbon substrate, is first consumed by catabolic enzymes that are constitutive (always present). After completion of translation, the B-galactosidase enzyme undergoes folding to form into an active enzyme that can breakdown lactose into its constituent monosaccharides. The messenger RNA code from the lac operon is translated by ribosomal RNAS to form lac-related enzymes, such as B-galactosidase. Glucose binds to a repressor protein that blocks the transcription of the lac operon. The now "open" operon is transcribed by RNA polymerase protein, resulting in messenger RNA code of the lac operon enzymes.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter28: Dna Metabolism: Replication, Recombination, And Repair
Section: Chapter Questions
Problem 21P
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Use the blanks on the left hand side to put the following statements in order (use numbers or letters to designate the order). All of the
statements refer to the diauxic (sequential) growth of a bacterium on the carbon substrates glucose and lactose (see textbook Fig 4.11 on
diauxic growth). Remember, the lac operon contains the genes necessary for the lactose catabolism.
The glucose substrate is completely consumed, at which time the repressor protein on the lac operon
unbinds from the DNA molecule.
The lactose substrate is catabolized by the active B-galactosidase enzyme.
Glucose, as the preferred carbon substrate, is first consumed by catabolic enzymes that are constitutive
(always present).
After completion of translation, the B-galactosidase enzyme undergoes folding to form into an active enzyme
that can breakdown lactose into its constituent monosaccharides.
The messenger RNA code from the lac operon is translated by ribosomal RNAS to form lac-related enzymes,
such as B-galactosidase.
Glucose binds to a repressor protein that blocks the transcription of the lac operon.
The now "open" operon is transcribed by RNA polymerase protein, resulting in messenger RNA code of the
lac operon enzymes.
Transcribed Image Text:Use the blanks on the left hand side to put the following statements in order (use numbers or letters to designate the order). All of the statements refer to the diauxic (sequential) growth of a bacterium on the carbon substrates glucose and lactose (see textbook Fig 4.11 on diauxic growth). Remember, the lac operon contains the genes necessary for the lactose catabolism. The glucose substrate is completely consumed, at which time the repressor protein on the lac operon unbinds from the DNA molecule. The lactose substrate is catabolized by the active B-galactosidase enzyme. Glucose, as the preferred carbon substrate, is first consumed by catabolic enzymes that are constitutive (always present). After completion of translation, the B-galactosidase enzyme undergoes folding to form into an active enzyme that can breakdown lactose into its constituent monosaccharides. The messenger RNA code from the lac operon is translated by ribosomal RNAS to form lac-related enzymes, such as B-galactosidase. Glucose binds to a repressor protein that blocks the transcription of the lac operon. The now "open" operon is transcribed by RNA polymerase protein, resulting in messenger RNA code of the lac operon enzymes.
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