Two sets of sequenced DNA fragments for question #13 (A) In this example, a DNA sequence of 150 bases is cut with two different restriction enzymes, producing the following fragments, each of which has been sequenced. Fragments from the first restriction enzyme: GGTCGGCTATGTAACGAGTTGCC тсттоттсстассттотсAлСССGGGAТGAATOTTTACTO САCCCGGACCGTсссттCAT GTCCCAGAGCстаттасалаллат GCCCACCTT TTATTGAGTTGATGCTCGACGTAGCCAGACTTAA Fragments from the second restriction enzyme: ACCGGGGATGAATGTTTACTGGTCGCAGAG ссТАттасаAGAAатсатсааста сттетсA TGATGCTCGACGT сотссаттсAт AGCCAGACTTAACACGCGGAC тоталсаAOттоссосСсАCсттттATталот тсттеттсстаG Try to piece these fragmentary sequences together and determine the entire sequence of 150 bases, before you turn the page. Figure 4-8a Biology Today, 3le (0 2004 Garland Science)
Two sets of sequenced DNA fragments for question #13 (A) In this example, a DNA sequence of 150 bases is cut with two different restriction enzymes, producing the following fragments, each of which has been sequenced. Fragments from the first restriction enzyme: GGTCGGCTATGTAACGAGTTGCC тсттоттсстассттотсAлСССGGGAТGAATOTTTACTO САCCCGGACCGTсссттCAT GTCCCAGAGCстаттасалаллат GCCCACCTT TTATTGAGTTGATGCTCGACGTAGCCAGACTTAA Fragments from the second restriction enzyme: ACCGGGGATGAATGTTTACTGGTCGCAGAG ссТАттасаAGAAатсатсааста сттетсA TGATGCTCGACGT сотссаттсAт AGCCAGACTTAACACGCGGAC тоталсаAOттоссосСсАCсттттATталот тсттеттсстаG Try to piece these fragmentary sequences together and determine the entire sequence of 150 bases, before you turn the page. Figure 4-8a Biology Today, 3le (0 2004 Garland Science)
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter19: Genomes And Proteomes
Section: Chapter Questions
Problem 1ITD: Below is a sequence of 540 bases from a genome. What information would you use to find the...
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Describe the process for shotgun sequencing of a genome. Practice aligning the two sets of sequenced fragments below, to determine the order of the fragments and the complete sequence.
![Two sets of sequenced DNA fragments for question #13
(A) In this example, a DNA sequence of 150 bases is cut with two different restriction
enzymes, producing the following fragments, each of which has been sequenced.
Fragments from the first restriction enzyme:
GGTCGGCTATGTAACGAGTTGCC
тсттоттсстассттотсAлСССGGGAТGAATOTTTACTO
САCCCGGACCGTсссттCAT
GTCCCAGAGCстаттасалаллат
GCCCACCTT
TTATTGAGTTGATGCTCGACGTAGCCAGACTTAA
Fragments from the second restriction enzyme:
ACCGGGGATGAATGTTTACTGGTCGCAGAG
ссТАттасаAGAAатсатсааста
сттетсA
TGATGCTCGACGT
сотссаттсAт
AGCCAGACTTAACACGCGGAC
тоталсаAOттоссосСсАCсттттATталот
тсттеттсстаG
Try to piece these fragmentary sequences together and determine the entire sequence
of 150 bases, before you turn the page.
Figure 4-8a Biology Today, 3le (0 2004 Garland Science)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fe8f91c38-09f5-4c00-bf6b-e8915c88bb3c%2Fbeaf1c52-8dd0-4140-9092-6a77bbbe6991%2Fj9oc5zl.png&w=3840&q=75)
Transcribed Image Text:Two sets of sequenced DNA fragments for question #13
(A) In this example, a DNA sequence of 150 bases is cut with two different restriction
enzymes, producing the following fragments, each of which has been sequenced.
Fragments from the first restriction enzyme:
GGTCGGCTATGTAACGAGTTGCC
тсттоттсстассттотсAлСССGGGAТGAATOTTTACTO
САCCCGGACCGTсссттCAT
GTCCCAGAGCстаттасалаллат
GCCCACCTT
TTATTGAGTTGATGCTCGACGTAGCCAGACTTAA
Fragments from the second restriction enzyme:
ACCGGGGATGAATGTTTACTGGTCGCAGAG
ссТАттасаAGAAатсатсааста
сттетсA
TGATGCTCGACGT
сотссаттсAт
AGCCAGACTTAACACGCGGAC
тоталсаAOттоссосСсАCсттттATталот
тсттеттсстаG
Try to piece these fragmentary sequences together and determine the entire sequence
of 150 bases, before you turn the page.
Figure 4-8a Biology Today, 3le (0 2004 Garland Science)
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