a PCR-based crime scene investigation, similar to the one presented in the lab module with Brother Y and Brother X, there is a sample of DNA from a crime scene that is likely to belong to the guilty party. Based on the gel photo below, which shows the results of an electrophoresis gel following PCR amplification at one locus of 5 DNA samples - one crime scene sample and 4 suspe

Q: COMPONENT 1 REACTION N+1 REACTIONS 15.80 µL 2.50 µL 1.50 µL 2.00 µL 1.00 µL 1.00 µL 0.15 µL…

A: Polymerase chain reaction is a procedure that is used to amplify a particular segment of DNA. This…

Q: PCR is a very useful method in biotechnology because it does not require the 6 or more different key…

A: PCR is a polymerase chain reaction- it ia process of replication of DNA in laboratory. PCR can…

Q: DNA extraction, PCR, gel electrophoresis, and DNA sequencing of PCR products. Give a description of…

A: DNA extraction is the procedure of isolating the Deoxyribonucleic acid from biological samples. PCR…

Q: A next-generation method of DNA sequencing, 454 sequencing, uses pyrosequencing in which the…

A: Pyrosequencing is a next-generation technique of DNA sequencing. This method can be used to detect…

Q: Identify the 4 components needed to start a PCR reaction (equipment not included)

A: Forensic researchers can look at DNA found at a crime location (from blood or hair, for instance) to…

Q: Which of the following is NOT a characteristic of PCR primers?  Short synthetic oligonucleotide…

A: Polymerase chain reaction is a procedure in which a strand of DNA is amplified by using short RNA…

Q: Which of the following is not a requirement for a PCR mix? O a. Taq polymerase O b. Excess…

A: Polymerase chain reaction Polymerase chain reaction or PCR , as it is commonly known who invented by…

Q: Which of the following correctly describes a possible scenario during a run of gel electrophoresis…

A: Agarose Gel electrophoresis(AGE) is a method used in molecular biology to separate mixed RNA, DNA,…

Q: You are interested in amplifying the putative gene encoding the 480-bp protein TMR (Total Memory…

A: We are given with the gene that is needed to be amplified by PCR. The image shows a region of DNA…

Q: Briefly explain the rationales of adding Bisacridine A which can affect DNA stability in polymerase…

A: PCR is used to enhance genes associated with patients' DNA genetic disorders. It has practical uses…

Q: What is the fastest method to determine if a genetic disorder is due to a mutation at a palindromic…

A: Mutations This refers to the alterations in the sequence of DNA. These are caused from the mistakes…

Q: a.What is the importance of centrifugation in step 3?  b.

A: Introduction: For the development of diagnostics and medications as well as for research into the…

Q: DNA Extraction Using household materials Wash the fruit. Place the fruit (e.g. banana) in a zip…

A: The molecular biology applications involve protein and nucleic acid isolations. The process involves…

Q: When you are extracting DNA one of the first steps is to dissolve the cell membranes with ice cold…

A: Gene expression: The process in which genetic information encoded in genes is expressed in the form…

Q: Make the PCR Cocktail This table lists the ingredients, stock reagent concentrations, and…

A: Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing…

Q: RECOMBINANT DNA BRIEFLY, DESCRIBE RECOMBINANT DNA AND GIVE ONE CONCRETE EXAMPLE. EVALUATE THE…

A: Through recombinant DNA technology, we can clone our desired gene into a plasmid to get the desired…

Q: A student carries out PCR using the following steps:  step 1: 94C for 1 minute Step 2: 60C for…

A: A complete set of chromosomes in any species is regarded as its genome. Each  chromosomes homes DNA…

Q: Why uncut and cut PCR at different position in the agarose gel? which one can be decided accuracy…

A: *Gel electrophoresis a technique to separate DNA fragments based on their size. *DNA samples are…

Q: suppose ampliTaq gold is being used to eliminate undesired amplicons in PCR. if the pH of the…

A: During PCR process amplicon is a stretch of DNA or RNA which is the source of amplification or DNA…

Q: annealing temperature

A: Answer:Annealing temperature depends the Tm of the complementary regions of your primer with the…

Q: Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA…

A: DNA extraction procedure refers to the methodology that is followed to purify the the DNA (cellular…

Q: Imagine that you correctly prepare your PCR reaction mixture, but there is something wrong with the…

A: The polymerase chain reaction is the PCR in which the genes are amplified i.e make millions to…

Q: Suggest and describe two methods to detect DNA concentration by using in text citation and provide…

A: DNA is genetic material in almost all living organism that transit from one generation to another

Q: When [ATP] is high and in excess, phosphocreatine (PCr) is made as an energy reservoir in muscle and…

A: About 90 percent of the body's creatine deposits are stored in the skeletal muscle.Phosphocreatine…

Q: In a PCR-based crime scene investigation, similar to the one presented in the lab module with…

A: The RAPD or rapid amplification of polymorphic DNA is used to detect the DNA that is present in the…

Q: Our PCR samples already contain loading dye, but sometimes this isn’t the case. If your samples…

A: Gel electrophoresis is a process that helps in the separation of DNA fragments based on sizes with…

Q: Please answer these two questions regarding PCR:   a) Why do you need to perform PCR on DNA obtained…

A: The polymerase chain reaction (PCR) is a technique used to make many copies of a specific segment of…

Q: You have a 100 micromolar stock solution of dNTPs in the freezer and are setting up a PCR reaction…

A: PCR is the polymerase chain reaction. In this exact amount of reagents to be added in order to…

Q: PCR is a powerful technique to screen and amplify segments of DNA for use in recombinant protein…

A: Introduction PCR is a method for amplifying a specific region of DNA from a mixed template. Reagents…

Q: Briefly describe the function of each of the following components of the DNA extraction protocol.…

A: Hi, Thanks For Your Question.  1. Function Of Heat In DNA Extraction : Answer : Heating Aids In The…

Q: Which of the following is required in running a PCR reaction? Promoter Primase DNTPS DNA polymerase…

A: PCR is polymerase chain reaction , which is a biotechnology tool used in DNA amplification.

Q: g best describes the complete sequence of steps occurring during every cycle of PCR? I-The primers…

A: A laboratory method used to make many copies of a specific piece of DNA from a sample that contains…

Q: Brenda is a junior student in the biomedical program at her school. She is starting the PCR genetic…

A: PCR is a polymerase chain reaction, by which a small segment, results in large and multiple…

Q: If you were to re-do this lab again with the same basic constraints (one DNA source, multiple…

A: DNA fragments are run on a agarose gel.

Q: Briefly explain the rationales of adding chemicals which can affect DNA stability in polymerase…

A: PCR is widely used to rapidly make millions to billions if copies of a specific DNA sample.

Q: In a Sanger sequencing-based PCR reaction, which molecules are more common:

A: The most important techniques available to the molecular biologist is the DNA sequencing by…

Q: step 4, isopropyl alcohol is used to precipitate out the DNA because it is not soluble. What does…

A: Introduction: Pure DNA is needed for DNA analysis. In cells, DNA can be found. However, cells also…

Q: You culture bacteria from the soil at a toxic waste dump on an agar plate and pick a single…

A: * Given that a culture of bacteria from soil of waste dump is collected and grown on agar plate and…

Q: Do you think it should be possible to convict criminals solely on gel electrophoresis of digested…

A: Yes, it would be possible to convict criminals solely on the basis of electrophoresis of digested…

Q: ook at each PCR component listed below.  For each one, determine which steps(s) of the PCR reaction…

A: PCR is the polymerase chain reaction in which a particular sequence is amplified. PCR requires many…

Q: Quantitative PCR differs from regular PCR in that it uses [A] to [B] the amount of [C] in a sample.…

A: PCR(polymerase chain reaction) is a molecular method to make multiple copies(amplify) of a small…

Q: Entire sequence below needs to be amplified by PCR and subcloned into a plasmid vector. Which of the…

A: PCR, or the polymerase chain reaction, is a technique used by molecular biologists to amplify…

Q: Identify three items (aside from water) that must be used in a Polymerase Chain Reaction (PCR) mi

A: Items that must be used in a Polymerase Chain Reaction (PCR) mixture are-   MgCl2. dNTPs. Taq…

Q: How we prepared the sample of DNA and what kinds of tracking dye we add to sample in SDS-PAGE ?…

A: INTRODUCTION SDS PAGE or Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis is a technique…

Q: You have added 4 μl of a 5 μM stock primer to a 25 μl PCR reaction. What is the amount of primer in…

A: To determine the amount of water the lyophilized primer is added and simply multiplied the number of…

Q: We usually think of enzymes as being most active at around 37°C, yet in PCR the DNA polymerase is…

A: PCR, like DNA replication in an organism, necessitates the use of a DNA polymerase enzyme to create…

Q: . Draw the melting curve for primer annealing, labeling the axes. Label the position of the…

A:

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.