Thermotolerant coliforms are often referred to as "faecal coliforms", although the organisms may not be of faecal origin. The time interval between collection of the sample and testing shoud not exceed 6 hours, why is this?
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Thermotolerant coliforms are often referred to as "faecal coliforms", although the organisms may not be of faecal origin. The time interval between collection of the sample and testing shoud not exceed 6 hours, why is this?
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- Describe the process of qualitative water test for coliform contamination. If shown EMB plates, be able to determine if coliforms are present or not.An otic product was subjected to AET and tested with the challenge microorganisms. An initial concentration of 2 x 10^5 cfu/mL of Escherichia coli was computed. Compute for the log reduction from the initial calculated count if the cfu/mL obtained is 1.2 x 10^4 at 7 days.The number of bacteria in saliva samples was determined by collecting the saliva, making serial dilutions, and inoculating nutrient agar by the pour plate method. The plates were inoculated aerobically for 48 hours at 37°C. What can you conclude from this data? Why? Mouthwash CFU per ml in salvia Before Use After Use Listerine 2.22 x 107 1.22x107 Schmidts Mouthwash 2.42 x 107 3.22x105 Biotene Mouthwash 3.21 x 107 2.12 x106
- In a(n) _________ test, the effectiveness of a microbial control agent is compared to that of carbolic acid. Question options: use-dilution in-use Kelsey-Sykes capacity phenol coefficient Entner-Doudoroff algebraWhat is the proper collection time period for a fecal fat specimen? 1) 24 hours 2) 48 hours 3) 72 hours 4) 96 hoursProve if the statement is TRUE or False with complete solution. At a dilution factor of, UHT-pasteurized milk sample X yielded the following bacterial colony counts when spread-plated in quadruplicates: 23, 20, 35, and 41 CFU/plate. As a result, the milk sample will pass laboratory standards if the total microbial limit is set to "not to exceed 30,000 CFU/mL of milk sample."
- In a Triple Sugar Iron test, what are the results and (what should they look like) for the bacteria: Enterobacter aerogenes, Pseudomonas aeruginosa, Salmonella typhimurium, and Serratia marcescens?Answer the following questions (not more than 5 sentences/question). Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.A stool specimen of a patient with severe diarrhea was cultured in a series of specialized media for isolation of enteric organisms. The cultures yielded three isolates that were species of Salmonella, Shigella, and Escherichia. Explain why the Hydrogen sulfide production test would be diagnostically significant.
- ORGANISMS: STENOTROPHOMONAS MALTOPHILIA Outline the presumptive and definitive characteristics for the laboratory identification of the bacterial species being presented. Discuss the diagnostic and clinical implications of making correct organism identification by giving follow up tests.Mary wanted to know the amount of microbes in her garden. To do this, she took a 10g soil sample, added urea, and then let it incubate for one week. She then performed serial dilution a shown below: 10 g soil sample To: 1ml 90 ml Dilution Bottle 1 ml 9 ml Tube 1 0.1 ml 0.45 ml 111 9.9 ml Tube 3 9 ml Tube 2 9.55 ml Tube 4 Calculate the dilution of the bottle and each tube. Be sure to show complete solutions and box your final answer.Discuss the correct laboratory technique in using the centrifuge for qualitative analysis. Give two tips on its proper use. Explain how calcium ions are confirmed present in a solid salt by using flame test. Discuss a method to confirm the presence of a) phenol and b) ketone in a test compound. How are proteins confirmed present in a sample? Explain the laboratory process.