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Why do we grow the bacteria in minimal media when testing for citrate utilization?
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- Yoghurt starter culture is a mixed population of S. thermophilus and L. bulgaricus, both competing for the same substrate, lactose. Is it possible for the two cultures to continue to co-exist?Mannitol salt agar contains salt to prevent the growth of most microbes and mannitol plus a pH indicator to detect mannitol fermentation. Which component of mannitol salt agar makes it a selective medium?Name six basic nutritional requirements supplied in all culture media.
- why is it important that a small amount of inoculum be used in preparation of an agar slant?MacConkey agar inhibits the growth of Gram-positive bacteria while encouraging the growth of Gram-negative bacteria. It also can determine if some bacteria use lactose. Define what type(s) of media MacConkey agar is, using as many terms in the answer list as are applicable.List all the possible reasons why the bacteria in broth suspension that were spread and pour plated are poorly grown.
- Why is agar ideal for microbial cultivation?What type of agar is MacConkey agar?A microbiologist used the concept of enrichment culture to isolate aerobic and anaerobic nitrogen-fixing bacteria, sulfate-reducing bacteria, and sulfur-oxidizing bacteria. What kind of selective media could he haveused for isolating each of these four classes of microbes?
- How can one distinguish E. coli from P.aeruginosa on nutrient agar? blood agar? EMB agar? What is the major difference between Thayer-Martin and chocolate agar? When would you use Thayer-Martin rather than chocolate agar?If an esculinase-postive and an esculinase-negative bacteria species can both grow on the Bile Esculin Agar media, how are the two bacteria species distinguished?What specifically causes colonies of lactose fermenters to be pink-red on MacConkey agar?