The write-up says “A C18-silica column is used in this experiment, a 0.46 x 15 cm column with 5-µm particles. Equilibrate the column with 20 empty column volumes of 0.010 M phosphate buffer (pH 7.2) at a flow rate of 1.2 mL/min before beginning chromatography.” Show the calculation for how much mobile phase you would use (volume) and how long it will take to equilibrate the column.

The write-up says “A C18-silica column is used in this experiment, a 0.46 x 15 cm column with 5-µm particles. Equilibrate the column with 20 empty column volumes of 0.010 M phosphate buffer (pH 7.2) at a flow rate of 1.2 mL/min before beginning chromatography.” Show the calculation for how much mobile phase you would use (volume) and how long it will take to equilibrate the column.

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter30: Capillary Electrophoresis, Electrochromatography, And Field-flow Fractionation

Section: Chapter Questions

Problem 30.11QAP

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