The investigators reported that the sphingosine kinase enzyme attached to the DEAE column when Tris was used as a buffer, but not when a phosphate buffer at the same pH was used. Explain the difference in behavior. The structure of the conjugate base of the Tris buffer is shown below. Hint: consider what charges all these molecules have at pH 7.4
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The investigators reported that the sphingosine kinase enzyme attached to the DEAE column when Tris was used as a buffer, but not when a phosphate buffer at the same pH was used. Explain the difference in behavior. The structure of the conjugate base of the Tris buffer is shown below. Hint: consider what charges all these molecules have at pH 7.4
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- Activity #8 Which protein(s) are retained on Ni²+-HiTrap Chelating HP? Self-assessment: not retained: retained: conalbumin drag to the left column those that are retained, to the right those that are not ferritin ribonuclease A thyroglobulin Hiss-luciferase glutathione reductase When done, assessExercise 3 The MTT assay is a widely used assay to study cell viability. It measures cellular metabolic activity, which serves an indicator of cell viability, proliferation and cytotoxicity. This non- radioactive colorimetric assay is based on the reduction of a yellow tetrazolium salt (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide or MTT) to purple formazan crystals by metabolically active cells. Viable cells contain NAD(P)H-dependent oxidoreductase enzymes that reduce the MTT to formazan. The insoluble formazan crystals are dissolved and the resulting coloured solution is quantified by measuring absorbance at 500-600nm using a multi-well spectrophotometer. The darker the solution, the greater the number of viable, metabolically active cells! Exercise 3.1 MTT protocol Your colleagues in the School of Biology and Environmental Science discovered an unusual looking green algae on a recent fieldtrip to Costa Rica. They have provided you with some extracts from the plant…Topic: Co-Enzyme Q10 Question: What does it mean when asked to explain the evidence behind medicinal chemistry of the CAM agent, and if the chemical structures relate to it's effects.
- BIOMOLECULES Please answer the questions properly. - Multiple choice Qyestion 1: If a cell has an adequate supply of adenine nucleotides but requires more guanine nucleotides for protein synthesis: 1. Glutamine-PRPP amidotransferase will not be fully inhibited. 2. AMP will be a feedback inhibitor of the condensation of IMP with aspartate. 3. ATP will stimulate the production of GMP from IMP. 4. ATP will inhibit nucleoside diphosphate reductase. А. 1, 2, and 3 В. 2 and 4 С. 1, 2, 3, and 4 D. 1 and 3PDB code 7BSJ Questions Q1 - What is the name of the protein Q2 - What does the protein do? Q3 - What are the structural features of the protein? Q4 - What are two features of your protein’s structure that make it different OR similar to haemoglobin? Q5 - Based on the structural properties of your protein, how resistant (or sensitive) would your protein be to heat denaturation and why?Work 3. The formation and secretion of hydrochloric acid Complete the scheme, specify the starting substances for the formation of HCI, the enzyme, the reaction product, the secreted substances and the substances entering the cell, specify the energy-consuming carrier. Substrates Enzyme Hydrochloric acid secretion is stimulated by: receptors - receptors - (ATP) energy-consuming transport O Carrier Channels receptors Prostaglandins E2 (PGE2) Img. 16. Secretory processes in the parietal cell (increase or inhibit?) gastric secretion. 2 Stomach cavity Interstitial fluid
- Super urgent: Explain why Histidine does not act as a basic acidic amino in general acid-base catalysis of lysozyme, which is at pH near 4.5? (His pka = 6.0)ACTIVITY NO. 10 MIXING STUDIES 1. Define Lupus Anticoagulants (LA's). 2. What could be the effects of LA's on the phospholipid dependent partial thromboplastin time (PTT) reaction? 3. What is the clinical significance of employing a moderate phospholipid or high - phospholipid PTT Reagent in their primary PTT assay? 4. Illustrate and label the different steps of Mixing Studies.Homework 1 (a) The kinetic data given below are for the reaction catalyzed by prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme. (b) Ibuprofen is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. Us ing the data in the first and third columns of the table, determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxi' Rate of formation [Arachidonic acid] (mm) Rate of formation of PGG2 (mm/min) of PGG, with 10 mg/mL ibuprofen (mM/min) 0.5 23.5 16.67 1.0 32.2 25.25 1.5 36.9 30.49 41.8 44.0 2.5 37.04 3.5 38.91
- #3.Answer needed quickly reponse.. After discussing his case with his physician, he learned that he had probably been the victim of pufferfish poisoning. The active toxin in the tissues of this fish is a chemical called tetrodotoxin (TTX). Tetrodotoxin is in a class of chemicals known as neurotoxins because it exerts its effects on neurons. The specific action of tetrodotoxin is that it blocks voltage-gated sodium ion channels. Define the following phrases and terms associated with the signs and symptoms of Dr. Westwood’s TTX poisoning: diaphoresis motor dysfunction paresthesias cyanotic hypoventilating bradycardia gastric lavage oxygen saturation As mentioned in the case description, tetrodotoxin is a molecule that blocks voltage-gated sodium ion channels. What is a voltage-gated sodium ion channel and what is its function?.Application (8) Long Answer Questions: Answer the following question(s) in complete sentences. 1) In Creutzfeldt-Jakob disease (the human form of mad cow disease), the brain rapidly deteriorates due to the build up of prions. Prions are proteins that have mutated into a form that is not water-soluble and that builds up in the brain. They are especially dangerous because they promote the refolding of native proteins. Explain why this refolding could be so detrimental to the cells in the brain, with reference to both structure and function of proteins. (Please answer fast and all Need only answer 1.A kinase is an enzyme that Answers A-D A .uses ATP to add a phosphate group to the substrate B .uses NADH to change the oxidation state of the substrate C.removes water from a double bond D.removes phosphate groups of substrates. 2.What is the right order for cascade of signal events Answers A-D A Effector proteins->Receptors-> Signal transducers B Signal transducers->Receptors->Effector proteins C Receptors >Signal transducers ->Effector proteins D Receptors-> Effector proteins->Signal transducers 3.PEP carboxykinase involve which of the following reaction Answers A-D A ATP to ADP B ADP to ATP C GDP to GTP D.GTP to GDP 4.Identify the phase of mitosis Answers A-D A Telophase B Anaphase C Prophase D Metaphase