Select one: a. continuous, batch, fermenter b. batch, continuous, chemostat c. batch, continuous, turbidostat d. batch, continuous, turbidostat e. continuous, batch, chemostat
Q: Describe the industrial fermentation process of Penicillin and Cephalosporin. Include points: -name…
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Q: Before inoculating and pouring molten nutrient agar into a plate, why must the agar first be cooled…
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Q: 300 +0,005 mg/mL - 0,010 mg/mL - 0,015 mg/mL +0,020 mg/mL 0,025 mg/mL + 0,030 mg/mL -0,035 mg/mL 250…
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Q: The bacterial cultures for the industrial fermentation process need to be maintained in an…
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Q: Which of the following agents (yeast, bacteria, and molds) do you think should be utilized to…
A: Yeast is a single-celled creature known as Saccharomyces cerevisiae that requires food, heat, and…
Q: Discuss the importance of some bacteria to undergo starvation during stationary phase and importance…
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Q: What is the key difference between reactions that result in the final product of fermentation to…
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A: The above topics are answered below
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Q: You record the following results of a nitrate reductase broth that was inoculated with an unknown…
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Q: how many bacteria/ml of the diluted broth would be present on average
A: 37 bacteria/ml of the diluted broth would be present on average.
Q: If a yellow halo is present around a colony on a mannitol salt agar (MSA) plate, the bacterium…
A: Mannitol salt agar (MSA) is used as a culture medium or growth medium. Growth medium is a medium…
Q: Why is the batch bioreactor is suitable in milk fermentation?
A: Batch bioreactor is a closed system and the process lasts until the nutrients are consumed.
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Q: James inoculated a species of bacteria into KIA media and realized that it has a yellow butt and a…
A: The correct Option of the answer for the question is Option A:-lactose fermenter The yellow slant…
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Q: Why do you think autoclave sterilization of media is preferred over open flame?
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Q: Which of the following statements regarding boiling is FALSE? Question options: all of these…
A: It is a multiple choice question.
Q: Which is statement below is the correct definition of the Sterility Assurance Limit (SAL). Select…
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Q: If a component is heat sensitive, how might you conveniently achieve sterility of that medium? a.…
A: Option A i.e. filtration
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Q: Briefly discussed the problems of fermentation scale up with respect to aeration, sterilization, and…
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Q: ar contains salt to prevent the growth of most microbes and mannitol plus a pH indicator to detect…
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Q: What is the batch reactor configuration/instruments in milk fermentation? How batch bioreactor is…
A: # Here I am giving answer of only first two questions . Please send questions one by one in the…
Q: Why do we grow the bacteria in minimal media when testing for citrate utilization
A: Minimal media are those that provide the minimal nutrients possible for colony development, usually…
Q: In the tripple sugar fermentation test, why is the triple sugar iron agar called a multi-test…
A: The process of chemical breakdown of complex substances into simple ones in the absence of oxygen…
Q: What type of media components cannot be sterilized in an autoclave?
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Q: The nutrient broth is a basic media used for growing a broad variety of microorganisms in the…
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Q: which of the following is not a chemical method of microbial control
A: Answer is option c - Filteration Filteration is not a chemical method of Microbial control.
Q: Microbial products can be classified as three major categories. With the use of sketch, briefly…
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Microbial fermentation can be carried out as either a once off ----------------- culture or a-------------------------- culture. The correct terminology for these respective cultures & the vessel the latter is carried out in are?
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- Microbial products can be classified as three major categories. With the use of sketch, briefly explain the kinetic patterns of growth and product formation in batch fermentation.Mannitol salt agar contains salt to prevent the growth of most microbes and mannitol plus a pH indicator to detect mannitol fermentation. Which component of mannitol salt agar makes it a selective medium?Menaquinones is produced by Flavobacterium meningosepticum via submerged fermentation with 60% working volume in 30-L fermentation. To obtain the crude menaquinones, the fermentation medium is centrifuged at 12,000 rpm for 15 minutes. The vacuum-freeze dried particles are crushed and collected. After that the samples are treated with methanol for 20 minutes. To remove polar lipid compounds from bacterial cells, HZ816 macroporous adsorption resin column is used with methanol-dichloromethane (l:1) as mobile phase and flow rate of 0.75 mL/min. Rotary evaporation at 500 C is carried out to concentrate the eluent. After that, the concentrated sample is purified with Bio-BeadsTM S-X3 to remove components without UV absorption. To obtain the homolog purified menaquinones, RP-C18 column is used. Recrystallization is carried out to obtain pure crystalized menaquinones. Answer the following: Indicate how to obtain the sample in the methanol solvent. Three different chromatography are used in…
- Assume you are the lead industrial microbiologist in a company that produces cholesterol-lowering fungal metabolite such as lovastatin. Your team isolated a novel fungus that can be used for large-scale production of lovastatin. Briefly describe how you would produce 20 L of this product from a potato dextrose agar plate culture using a 20 L fermentor.Describe the industrial fermentation process of Penicillin and Cephalosporin.Include points: -name of strain used as inoculum-type of fermentation process (batch/fed-batch/continuous) and bioreactor (airlift/ continous stirred Tank/ any other) used-media preparation (carbon source, nitrogen source, salts, oxygen requirement)-pH and remperature maintainedDescribe how a Triple Sugar Iron agar tube would appear if the following situation was true. Please note that all items in the following list are occurring in the same tube of agar. 1. Positive for glucose fermentation 2. Negative for lactose and sucrose fermentation 3. Positive for H2S production 4. Negative for gas production
- The thermal death point is best described as:(a) The highest temperature that will support growth of amicrobial culture(b) The length of time required for killing 100% of mi-crobes in a 24-hour culture that is heated to 100°C(c) The temperature at which a previously growing 24-hourculture will begin to die(d) The temperature at which 100% of the microbes in a24-hour culture will be killed in 10 minutesWhat is the purpose of adding mineral oil to an O/F Media tube and why do we only put mineral oil in only one? Which sugars are typically tested in the phenol red broth? Can the phenol red test be also used to determine if a certain bacteria can metabolize various carbohydrates? Explain how conducting the MR/VP test too soon in a culture’s growth cycle can lead to false negatives? Generally, the color change we see in these tests is a result of what change in the media? Explain. What is the MR-VP results for coli and E. aerogenes?For each of the following situations, describe how bacteria on these growth. media would appear on the plate: A. On MacConkey agar, a bacterium that grows on the medium but is negative for lactose fermentation B. On EMB agar, a bacterium that grows on the medium but is positive for lactose fermentation C. On mannitol salt agar, a bacterium that failed to grow on the medium D. On mannitol salt agar, a bacterium that grew on the medium and fermented mannitol
- What is the key difference between reactions that result in the final product of fermentation to form lactic acid or alcohol? What significance do these differences have in terms of microbial technology?Of what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? What precautions should be taken to control laboratory contaminants? Of what advantage is the using a solid and a liquid medium?The nutrient broth is a basic media used for growing a broad variety of microorganisms in the laboratory. The nutrient broth consists mainly of 1.5g * L ^ - 1 extract, 3g * L ^ - 1 yeast extract and 5g * L ^ - 1 sodium chloride dissolved in distilled water. The nutrient agar is prepared by adding the agar at the desired amount into nutrient broth. Answer the following questions. marks) a) How would you prepare a 500 ml nutrient broth? b) How would you prepare 2% (w / v) nutrient agar for 1L? c) How would you prepare a 100 ml nutrient broth supplemented with 100mu * g / m * l ampicillin antibiotic? The stock concentration for ampicillin solution is 100mg / m * l .