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. Rotavirus, a disease-causing member of the Reovirus family (with double-stranded RNA, but no DNA), must use which of the following enzymes to replicate its
- RNA-dependent-RNA-polymerase
- RNA-dependent-DNA-polymerase
- DNA-dependent-RNA-polymerase
- DNA-dependent-DNA-polymerase
- reverse transcriptase
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- A person is infected with HIV. The individual is treated with several drugs, including azidothymidine (AZT). Which of the following is the most immediate effect of this drug? O It inhibits formation of the capsid. O It does not allow the viral genome to become integrated into the host genome. O It allows reverse transcriptase to proofread, and this will allow successful treatment with a vaccine. O It does not allow the virus to make a DNA copy of its genome.Which step(s) in the replication cycle of HIV can still occur in the presence of a reverse transcriptase inhibitor? Select all that apply. transcription of viral genes initial viral attachment viral assembly integration of viral DNA into host genome initial viral entryCoronaviridae is a virus “family.” There are actually many different coronaviruses within this group, including less pathogenic virus strains that cause the common cold. Many people in this course have likely been infected with one of these other coronaviruses. A common feature of coronaviruses and other RNA viruses (think influenza virus, HIV, etc.) is that they mutate frequently. This is because the viral enzymes that copy the genome during replication can’t fix mistakes. Given this information, how might we be able to distinguish the SARS-CoV-2 strain from some of the other, less concerning, coronavirus strains?
- Viruses can easily mutate exchange genetic material, mechanisms referred to as antigenic drift and antigenic shift. These changes allow the virus to evade the immune system defenses. Match each term with the correct definition Antigenic drift [ Choose ] [Choose ] Different strains of a virus or strain of different viruses, combine and form a new strain that now has a mixture of the surface antigens Antigenic shift The virus changes genetically by accumulating mutations within the genes that code for antibody-binding sitesMatch each of the following replication classes of viruses with the one description that best describes it. This virus class is composed of segmented and non-segmented helical viruses that carry an RdRp within the virion into the cell. Example: Rabies virus These viruses are non- enveloped, icosahedral, have segmented genomes, and carry their own RNA-dependent RNA polymerase. The viral genome is copied following assembly. Example: Reovirus This is the only class of RNA viruses whose genome is replicated by cellular enzymes and not an RNA-dependent RNA polymerase. Cellular DNA polymerases replicate this virus's non- integrated genome, although transcription is not the initial event that takes place in the process. Example: Parvoviruses In this class of viruses, transcription occurs before genome replication because viral proteins are involved in replicating the genome. Example: Adenovirus This class of viruses forms circular DNA and creates pre- genomic RNA using host RNA polymerases.…The genome of which of the following types of animal virus can act directly as MRNA and be utilized by ribosomes without prior processing? O 1) retroviruses O 2) SSDNA viruses 3) -SSRNA viruses O 4) +SSRNA viruses 5) dsDNA viruses
- Which of the following is a known characteristic that can lead to genetic changes in some viruses? Operons in the viral genome can undergo homologous recombination, leading to genetic rearrangements. Reassortment of segmented genomes can occur if a host cell is co-infected with two different viral strains. RNA polymerases used in replication of the viral genome have proofreading capability. Environmental conditions can trigger the SOS response, which triggers mutations in the viral genome.Arrange the following list into the correct sequence for part of the cycle of a retrovirus: 1. dsDNA integrated into host DNA 2.viral proteins synthesized on host ribosomes 3. viral DNA uses host enzymes to transcribe viral RNA 4. reverse transcriptase catalyzes synthesis of ssDNA 5. synthesis of second DNA strand (a) 5, 2, 1, 3, 4 (b) 5, 2, 3, 4, 1 (c) 4, 5, 1, 3, 2 (d) 4, 1, 2, 3, 5 (e) 2, 1, 3, 4, 5An antimicrobial drug binds to the HIV reverse transcriptase enzyme, preventing it from working. Which of the following is true of this drug? It would inhibit early replication steps of some viruses, but not affect normal eukaryotic cell activity. It would directly interfere with translation of some viral proteins and some eukaryotic proteins. It would block assembly/maturation steps of some viruses, and interfere with translation in eukaryotic cells. It would interfere with release of newly formed viruses from a host cell, but not affect eukaryotic cells. It would interfere with protein synthesis in bacteria, but not affect eukaryotic ribosomes. It would interfere with mRNA transcription in bacteria and viruses, but not affect eukaryotic transcription.
- Retroviruses are dependent on reverse transcriptase, which does which of the following? Transcribes RNA backward (3' to 5' instead of 5' to 3') None of these are correct. Creates transcripts from an RNA template Creates a DNA copy of an RNA template.Reverse transcriptase is an error prone DNA polymerase. Describe the role of reverse transcriptase in viral propagation and how having an error-prone enzyme might make it challenging to have a vaccineWhich of the following statements are correct for a class V ssRNA (-) virus? it uses its genome directly for translation it must make the complementary (+) RNA strand to synthesize more of its genome it replicates its genome via classical semiconservative replication it converts its genome to DNA via reverse transcriptase as part of its life cycle