Question 13 Listen The closer two genes are together on a chromosome, the fewer recombinants are found between them a) True b) False
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Genetics Question 13
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- QUESTION 11 "Waxy endosperm (wx), virescent seedling (v), and shrunken endosperm (sh) are all recessive mutants in corn. Waxy and shrunken are both on chromosome 9, located 18 map units apart. Virescent is on chromosome 5 and is not linked to the other loci. A strain of corn that is homozygous for waxy and virescent is crossed to a strain that is homozygous for shrunken endosperm. The F1 were entirely wild type. An F1 individual was then test crossed to a strain that is homozygous for waxy, virescent and shrunken. Of 1000 offspring, how many of the offspring will be virescent and shrunken, but not waxy?" 205 430 230 215 200Question 17 What is the nucleotide sequence of the DNA strand that is complementary to 5-АTCGCAACTGTCAСТА-3 A 5-UAGUGACAGUUGCGAU-3' B 5'-TAGCGTTGACAGTGATA-3" с) 5-АТCАСTGTCААCGCTA-3 D 5'-TAGTGACAGTTGCGAT-3'Question 17 What is the nucleotide sequence of the DNA strand that is complementary to 5'-ATCGCAACTGTCACTA-3 A 5'-UAGUGACAGUUGCGAU-3' B 5-TAGCGTTGACAGTGATA-3 5'-ATCACTGTCAACGCTA-3 D 5'-TAGTGACAGTTGCGAT-3'
- Question 4 In order to replicate both strands of DNA SIMULTANEOUSLY, E. coli bacteria folds or loops one strand so both strands can do 5' to 3' synthesis. (A True B FalseQUESTION 7 After you graduate, you are working at a laboratory that identifies a novel protein called Leprechaun. To determine in which organelles Leprechaun is found, you decide to express the Leprechaun gene in HeLa cells using a plasmid. Since you have generated an antibody that recognizes an epitope within the Leprechaun protein, which technique will you use to identify its localization within the HeLa cells? OA. SDS-PAGE O B. Immunocytochemistry O C. Western blot OD. Transmission electron microscopy (TEM) OE. Immunohistochemistry QUESTION 8 Which of the following statements regarding electron microscopy is TRUE? OA. White light is utilized to illuminate the sample for imaging in all types of electron microscopy. OB. Electrons pass through the object being examined in transmission electron microscopy (TEM). OC. In scanning electron microscopy (SEM) electrons, electrons bounce off the surface of the sample. OD. Electron microscopy can be used to view structures as small as 2 nm in…Question 14 Friedreich's ataxia is a rare inherited disease that causes nervous system damage and movement problems. It usually begins in childhood and leads to impaired muscle coordination (ataxia) that worsens over time. Friedreich's ataxia is caused by an insertion of several copies of a three nucleotide sequence in a gene called FXN. Which genetic testing method would be most appropriate for testing for Freidreich's ataxia? O PCR with gel electrophoresis O PCR with conventional sequencing O PCR with SNP-chip O PCR with whole-genome sequencing
- Question 23 Both pseudogenes and orthologous genes are duplicated in the genome of the same species. Paralogous genes are gene duplication that acquire new/specialized function. The 1st statement is True and the 2nd statement is False. O The 1st statement is False and the 2nd statement is True. O Both Staments are True. O Both statements are False.Question 15 Only eukaryotic transcription enzymes will synthesize small nuclear RNA molecules involved in the maturation and processing of other RNAS. (A) True B) FalseQUESTION 17 OL NEere interested in generating a PCR amplicon including the bracketed sequence below. Which of the following sequences would be canen hybridizing (annealing) with the target AND would also serve to generate a copy of the bracketed region of interest? 5'-AATCGT[AGCAGCAGCAGTGGCT]A AGCT-3 3' -TTAGCA[TC GTC GTC GTC ACC G A] TTCG A - 5' 3-TTAGC-S S-AATCG-3 OSAAGCT-3 5-AGCTT-3 5-GCTAA-3 5-TCGAA-3 QUESTION 18 Vhich of the following is/are true regarding the enzvme PRIMASE? Save and Submit to save and submit. Click Save All Answers to save all answers.
- QUESTION 6 To verify the is indeed inside your plasmid, you'd like to do a colony PCR. But you need primers for your reaction. Which of the following primer pairs would probably work for verifying your insert is actually present in the plasmid? 5' ATGTTTATTTTCTTATTATTTTTTACTCTCACTAGTGGTAGTGACCTTGACCGGTGCACCACTTTTGATG ATGTTCAAGCTCCTAATTACACTCAACATACTTCATCTATGAGGGGGG TTTACTATCCTGATGAAATTTT (Very long, but a bunch of nucleotides her e).... TCTTGCTTTGTTGCATGACTAGTTGTTGCAGTTGCCTCAAGGGTGCATGCTCTTGTGGTTCTTGCTGCAA GTTTGATGAGGATGACTC TGAGCCAGTTCTCAAGGGTGTCAAATTACATTACACATAA 3' Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm= 59.8 O A. Reverse: 5' CAA ATT ACA TTA CAC ATA A 3' Tm= 47.4 Forward: 5' ATG TTT ATT TTC TTA TTA TTT 3' Tm= 47.1 C O B. Reverse: 5' TAT GTG TAA TGT AAT TTG ACA CCC 3' Tm3 58.4 Forward: 5' GGT AGT GAC CTT GAC CGG 3' Tm3 59.8 OC. Reverse: 5' GTG TAA TGT AAT TTG ACA CCC TTG 3' Tm: 59.4 Forward: 5' GGT CAC TAC CAC TAG TGA GAG 3' 59.4 C O D. Reverse: 5' GTG TAA TGT AAT TTG ACA CCC TTG…Question 22 Cloning vector contains a multiple cloning site (MCS) or polylinker containing multiple unique restriction sites. It also contains markers that allow for rapid selection of colonies with insert-containing plasmids in them. The 1st statement is True and the 2nd statement is False. The 1st statement is False and the 2nd statement is True. O Both Staments are True. O Both statements are False.Question 18 Recessive mutations in autosomal genes purple eye (pr), curved wing (c) and black body (b), as well as their wild type alleles are used for genetic mapping. An F1 Drosophila female heterozygous for purple eye (pr), curved wing (c) and black body (b) is crossed to a homozygous mutant male. This is a standard three-point mapping cross. The observed numbers and phenotypes of of the offspring from this cross are follows: 360 pr c b 380 pr+ c+b+ 107 pr c+ b 98 pr+ c b+ 27 prcb+ 18 pr+ c+ b 6 pr c+b+ 4 pr+ c b A total of 1000 flies. What is the distance between genes pr and b? 21.5 m.u. 1 m.u. 5.5 m.u. 6 m.u. 21 m.u. Qu