PURPLE VESTIGIAL DIHYBRID TESTCROSS In the parental generation, you mate a pure-breeding wild-type female (put/put.vg+/vg+) with a pure-breeding purple, vestigial (pu/pu;vg/vg) to produce an F1 generation that is all wild-type (pu*/pu;vg+/vg). Note that the F1 flies are all dihybrid. Next, you mate several F1 dihybrid females (put/pu;vg+/vg) with tester males, which are purple, vestigial (pu/pu;vg/vg). The offspring of this dihybrid testcross are: Phenotype Wild-type Purple, vestigial Vestigial Purple Genotype Tester Gamete Dihybrid Gamete Number 437 417 77 59 Copy the table into your notes and derive the dihybrid gametes following the example in the first section. The columns in blue (phenotypes and numbers of offspring) are what you can see and count. The genotypes of the testcross offspring (orange) must be deduced from the phenotypes and knowing that the tester contributed pu vg gametes. Finally, you can deduce the dihybrid gametes (green) by subtracting the tester gamete contribution from the offspring genotypes. Note that the two parental gamete types (+ + and pu vg) are the most abundant, as expected. Use the data to calculate the recombination frequency and the genetic map distance between the two genes. Record the map distance in your notes.
PURPLE VESTIGIAL DIHYBRID TESTCROSS In the parental generation, you mate a pure-breeding wild-type female (put/put.vg+/vg+) with a pure-breeding purple, vestigial (pu/pu;vg/vg) to produce an F1 generation that is all wild-type (pu*/pu;vg+/vg). Note that the F1 flies are all dihybrid. Next, you mate several F1 dihybrid females (put/pu;vg+/vg) with tester males, which are purple, vestigial (pu/pu;vg/vg). The offspring of this dihybrid testcross are: Phenotype Wild-type Purple, vestigial Vestigial Purple Genotype Tester Gamete Dihybrid Gamete Number 437 417 77 59 Copy the table into your notes and derive the dihybrid gametes following the example in the first section. The columns in blue (phenotypes and numbers of offspring) are what you can see and count. The genotypes of the testcross offspring (orange) must be deduced from the phenotypes and knowing that the tester contributed pu vg gametes. Finally, you can deduce the dihybrid gametes (green) by subtracting the tester gamete contribution from the offspring genotypes. Note that the two parental gamete types (+ + and pu vg) are the most abundant, as expected. Use the data to calculate the recombination frequency and the genetic map distance between the two genes. Record the map distance in your notes.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:PURPLE VESTIGIAL DIHYBRID TESTCROSS
In the parental generation, you mate a pure-breeding wild-type female (put/put.vg+/vg+) with a pure-breeding purple, vestigial
(pu/pu;vg/vg) to produce an F1 generation that is all wild-type (put/pu;vg+/vg). Note that the F1 flies are all dihybrid.
Next, you mate several F1 dihybrid females (put/pu;vg+/vg) with tester males, which are purple, vestigial (pu/pu;vg/vg). The offspring of
this dihybrid testcross are:
Phenotype
Wild-type
Purple, vestigial
Vestigial
Purple
Genotype Tester Gamete Dihybrid Gamete Number
437
417
77
59
Copy the table into your notes and derive the dihybrid gametes following the example in the first section. The columns in blue (phenotypes
and numbers of offspring) are what you can see and count. The genotypes of the testcross offspring (orange) must be deduced from the
phenotypes and knowing that the tester contributed pu vg gametes. Finally, you can deduce the dihybrid gametes (green) by subtracting the
tester gamete contribution from the offspring genotypes.
Note that the two parental gamete types (+ + and pu vg) are the most abundant, as expected. Use the data to calculate the recombination
frequency and the genetic map distance between the two genes. Record the map distance in your notes.
Manning Exercise 1c.
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