purification of egg yolk vitelline 1) Measure the volumes of 3 egg yolks and mix by adding an equal volume of NaCl solution. 2) Extract the mixture with 3 solid volumes of ether and separate the aqueous phase. 3) Do the same procedure 3 times. 4) Mix the sample with water and rinse. 5) It is expected for the protein to collapse. 6) Some more water is added in order to check whether the collapse has occurred completely. 7) The sample is centrifuged and the precipitate is dried.For each step, explain why that action was taken. for example why is NaCl added? Why is the procedure repeated 3 times?
purification of egg yolk vitelline 1) Measure the volumes of 3 egg yolks and mix by adding an equal volume of NaCl solution. 2) Extract the mixture with 3 solid volumes of ether and separate the aqueous phase. 3) Do the same procedure 3 times. 4) Mix the sample with water and rinse. 5) It is expected for the protein to collapse. 6) Some more water is added in order to check whether the collapse has occurred completely. 7) The sample is centrifuged and the precipitate is dried.For each step, explain why that action was taken. for example why is NaCl added? Why is the procedure repeated 3 times?
Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter22: Proteins
Section: Chapter Questions
Problem 22.101P: 22-101 Using what you know about protein denaturation, what is one reason you must maintain a body...
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purification of egg yolk vitelline
1) Measure the volumes of 3 egg yolks and mix by adding an equal volume of NaCl solution.
2) Extract the mixture with 3 solid volumes of ether and separate the aqueous phase.
3) Do the same procedure 3 times.
4) Mix the sample with water and rinse. 5) It is expected for the protein to collapse. 6) Some more water is added in order to check whether the collapse has occurred completely. 7) The sample is centrifuged and the precipitate is dried.For each step, explain why that action was taken. for example why is NaCl added? Why is the procedure repeated 3 times?
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