Using reversed phase chromatography in order to separate chloroquine and hydroxychloroquine. Which will be the first one to elute out of the column? Or both will elute the same time?
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Using reversed phase chromatography in order to separate chloroquine and hydroxychloroquine. Which will be the first one to elute out of the column? Or both will elute the same time?
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- In reverse phase chromatography which analyte will elute first, most polar or least polar?Which is the correct order of elution of a gas chromatography that has a polar column that contains water, acetone, and n-propanol.what is chromatography? Explain different types of chromatography and explain how to seperate compounds. indicate stationary phase mobile phase.
- 6) You are given a mixture of organic compounds suspected of containing various amines (e. g. 4-chloroaniline). What is the best way to quickly separate these amines from the other organic compounds? a) Perform a fractional distillation on the mixture. b) Benchtop silica gel column chromatography. c) Dissolve the mixture in an organic solvent and treat the solution with aqueous HCI. d) Dissolve the mixture in an organic solvent and treat the solution with aqueous NaOH.a) What would you see if you ran a Thin Layer Chromatography (TLC) on a mixture of the three solids shown below using silica gel as the stationary phase and ethyl acetate/hexanes as the mobile phase? Whichsolid would have the highest Rf and which would have the lowest Rf? Explain b) Would changing the mobile phase (from part a) and increasing thepolarity by introducing small amount of methanol to the mobile phase change the order ofRf of the above 3 compounds? Explain c) Another type of chromatography is Reverse Phase Chromatography. In this type ofchromatography, the stationary phase is non-polar and the mobile phase is polar. If astudent performed Reverse Phase TLC on the mixture of 3 solids (from part a),what would be the order of Rf’s of the compounds? Which would have the lowest Rf andwhich would have the highest Rf?In paper chromatography, a rectangular piece of filter paper serves as the mobile phase stationary phase eluent solute sample spots
- Using a normal phase chromatography, which solute will elute out first from column? Chlorophyll Pheophytin O B-carotene XanthophylsThe surface of the silica gel has a structure that looks like this diagram below. Suppose you used a plate coated with silica gel, with propanone, CH3COCH3, as the solvent for thin-layer chromatography. Suppose also that the mixture you were trying to identify contained benzaldehyde benzanilide phenol 1) rank the Rf value from large to small 2) name the IMF between each compound and the silica gel OH OH OH -0-Si-O-Si-O-Si-O- O main body of silica structureThe surface of the silica gel has a structure that looks like this diagram below. Suppose you used a plate coated with silica gel, with propanone, CH3COCH3, as the solvent for thin-layer chromatography. Sno Suppose also that the mixture you were trying to identify contained • benzaldehyde •benzanilide • phenol 1) rank the Rf Value from large to small 2) name the IMF between each compound and the silica gel OH OH main body of silica structure
- Suppose that you try separating an unknown mixture by normal-phase chromatography with the solvent mixture 50% hexane-50% methyl t-butyl ether (which is more polar than hexane). The peaks are too close together and are eluted rapidly. Should you use a higher or lower percentage of hexane in the next run?Look up the boiling point for each of the following compounds then determine the order that they will elute from the column. Use this elution order to identify the compound in each of the 6 standard chromatograms. Once you have identified the compound in each chromatogram, record its retention time. Compound Structure Compound Name Elution Order (1-6) Non polar → Polar Boiling Retention Time (From Chromatograms) Point Propyl acetate H H. H. Butyl acetate H. H. H H. H H. H. H. Methanol H O- Ethanol 2-butanone H H H. H. 4-methyl-2- pentanone H. H. HH H.Place the compounds in the order they will elute from the column when separated using reverse-phase liquid chromatography (RPLC). CH3 CH3 p-xylene napthalene CH3 anisole OH phenol anisole p-xylene Elutes first Elutes last Answer Bank naphthalene phenol