Preparation of a Calibration Curve Standard Bovine Serum Albumin (BSA) solution Stock solution: 2.00 mg/mL Standard solutions: 1.00 mL of 0, 0.125, 0.25, 0.5, 1.0, 1.5 and 2.0 mg/mL BSA Diluent: distilled water a. on the picture b.Using a micropipette, obtain 62.5 μL of 2.00 mg/mL stock BSA solution, then add 937.5 μL of distilled water. Mix using a vortex mixer. c. Complete Table 2.4 on your own. Calculate the volumes needed for preparing 1.00 mL of the remaining concentrations following the sample calculation. Table 2.4. Volumes needed for the preparation of the BSA standard solutions. Concentration to be prepared, mg/mL Volume of 2.00 mg/mL stock solution needed, μL Volume of distilled water needed, μL 0.00 0 1000.00 0.125 62.5 937.5 0.25 0.50 1.00 1.50 2.00 please complete the given table with the correct computation like in the picture
Preparation of a Calibration Curve Standard Bovine Serum Albumin (BSA) solution Stock solution: 2.00 mg/mL Standard solutions: 1.00 mL of 0, 0.125, 0.25, 0.5, 1.0, 1.5 and 2.0 mg/mL BSA Diluent: distilled water a. on the picture b.Using a micropipette, obtain 62.5 μL of 2.00 mg/mL stock BSA solution, then add 937.5 μL of distilled water. Mix using a vortex mixer. c. Complete Table 2.4 on your own. Calculate the volumes needed for preparing 1.00 mL of the remaining concentrations following the sample calculation. Table 2.4. Volumes needed for the preparation of the BSA standard solutions. Concentration to be prepared, mg/mL Volume of 2.00 mg/mL stock solution needed, μL Volume of distilled water needed, μL 0.00 0 1000.00 0.125 62.5 937.5 0.25 0.50 1.00 1.50 2.00 please complete the given table with the correct computation like in the picture
Chapter13: Dimensional Analysis/units Conversion
Section: Chapter Questions
Problem 1.10P
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Question
2.4. Preparation of a Calibration Curve
Standard Bovine Serum Albumin (BSA) solution
Stock solution: 2.00 mg/mL
Standard solutions: 1.00 mL of 0, 0.125, 0.25, 0.5, 1.0, 1.5 and 2.0 mg/mL BSA
Diluent: distilled water
a. on the picture
b.Using a micropipette, obtain 62.5 μL of 2.00 mg/mL stock BSA solution, then add 937.5 μL of
distilled water. Mix using a vortex mixer.
c. Complete Table 2.4 on your own. Calculate the volumes needed for preparing 1.00 mL of the
remaining concentrations following the sample calculation.
Table 2.4. Volumes needed for the preparation of the BSA standard solutions.
| Concentration to be prepared, mg/mL |
Volume of 2.00 mg/mL stock solution needed, μL |
Volume of distilled water needed, μL |
| 0.00 | 0 | 1000.00 |
| 0.125 | 62.5 | 937.5 |
| 0.25 | ||
| 0.50 | ||
| 1.00 | ||
| 1.50 | ||
| 2.00 |
please complete the given table with the correct computation like in the picture
Transcribed Image Text:a. Calculate the volume of stock solution needed. Use C.V1=C2V2.
Sample calculation:
How to prepare 1.00 mL of 0.125 mg/mL BSA solution from a 2.00 mg/mL stock solution?
Use CVi=C2V2
where CVi is the concentration and volume of the stock solution,
and CV2 is concentration and volume of the solution to be prepared.
So,
V1=(C¿V2)/C,
V;=(0.125 mg/mL x 1.00 mL)/2.00 mg/mL = 0.0625 mL = 62.5 µL of 2.00 mg/mL
BSA
ORATORY
try and Agri
Volume of distilled water = 1000 µL - 62.5 µl = 937.5 µL
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