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Basic Clinical Laboratory Techniques 6E

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter3: Basic Hemostasis

Section3.6: D-dimers

Problem 8RQ

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Predict the amino acid sequence based on the conditions below. Use the one-letter abbreviation of amino acids.
___1___--_2_-
3
--
--____6_--_7__
a. The amino acid in the C-terminal is optically inactive.
b. The fifth amino acid is a neutral aromatic amino acid that yields a negative result for the Xanthoproteic test using intact protein but produces a yellow soln with basic hydrolysate. It also gives a negative
result with both Hopkin's Cole and Millon's test using basic hydrolysate.
c. After treatment of this peptide with aminopeptidase (specificity: peptide bond on the N-terminus), it yields serine and a hexapeptide
d. The amino acid besides the N-terminal amino acid. will yield ornithine and urea after alkaline hydrolysis.
e. The third amino acid gives a positive result for Hopkins-Cole Test.
f. Chymotrypsin^ cannot cleave the peptide bond on the fifth amino acid because of the presence of this a.a. residue.
g. The amino acid at the carboxy-end of the third amino acid will yield a black precipitate with Pb(Ac)2 reagent.
*Chymotrypsin is an enzyme that cleaves the peptide bond at the carboxyl end of an aromatic amino acid except when proline is bonded to it

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