Net Absorbance (595 nm) Which protein standard curve would you use to read off the protein concentration of an unknown sample with an absorbancy value 175 150 125 .00 0.75 0.50 0.25 BSA BGG 0.00 500 1,000 1,500 2,000 Protein Concentration (pg/ml) Oouad imn BSA standard curve O BGG standard curve O Ether BSA or BGG standard curves O Neither BSA or BGG standard curves QUESTION 2

Net Absorbance (595 nm) Which protein standard curve would you use to read off the protein concentration of an unknown sample with an absorbancy value 175 150 125 .00 0.75 0.50 0.25 BSA BGG 0.00 500 1,000 1,500 2,000 Protein Concentration (pg/ml) Oouad imn BSA standard curve O BGG standard curve O Ether BSA or BGG standard curves O Neither BSA or BGG standard curves QUESTION 2

Organic Chemistry

8th Edition

ISBN:9781305580350

Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Publisher:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. Foote

Chapter20: Dienes, Conjugated Systems, And Pericyclic Reactions

Section: Chapter Questions

Problem 20.26P

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q41 IF YOU DO NOT KNOW THE ANSWER PLEASE DO NOT REJECT IT please calculate the unknown concentration of the protein D wih an absorbance value of A412 given the standard curve indicated in the table. write your answers in numbers only with 2 decimals. protein concentration (ug/ml) absorbance 0 0.000 0.02 0.161 0.04 0.284 0.06 0.438 0.08 0.572 0.10 0.762
You obtained the following raw data when setting up a Biuret standard curve: Absorbancy 540nm BSA (mg/ml) 0.158 1 0.210 2 0.260 3 0.305 4 0.360 0.410 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.279 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
The concentration and absorbance data below were collected for compound X. A sample of compound X of unknown concentration has a corrected average absorbance of 0.5621 (3 replicate measurements). Calculate the y-intercept. CONCENTRATION (mg/mL) ABSORBANCE 5 0.422 10 0.510 15 0.621 a. 0.299 b. 0.222 c. 0.319 d. 0.553
Pre-Lab Questions 1. Define a protein, and describe one important function of proteins in the human body. 2. The following data were obtained from a Bradford assay to determine the protein content in a sample: Protein content (ug) Absorbance value 0 0.000 2 0.022 5 0.065 10 0.106 20 0.178 30 0.299 40 0.380 50 0.472 unknown 0.150 Use Excel, another program, or graph paper to produce a calibration curve based on the data. Use linear regression (or best fit line) to obtain the slope of the line. Determine the unknown protein content for a sample that shows an absorbance reading of b. С. 0.150.
You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) Absorbancy 540nm 0 0.158 1 0.210 2 0.260 3 0.305 4 0.360 5 0.410 6 0.455 7 0.510 8 0.530 9 0.550 10 0.554 After blanking against a biuret-dH2O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.251 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).
The concentration and absorbance data below were collected for compound X. A sample of compound X of unknown concentration has a corrected average absorbance of 0.5621 (three replicate measurements). Calculate the y-intercept? Concentration Absorbance (mg/mL) 0.422 10 0.510 15 0.621 0.299 0.222 0.319 O 0.553
A direct ELISA experiment was done to determine the amount of protein present in a sample. A standard curve was prepared by plotting log Absorbance versus log concentration of protein in (ng/mL). The equation of the line is Y= 0.895 X -1.25. If an unknown protein shows an absorbance of 1.23. Calculate the concentration of this protein in the sample in (ng/mL).
* Question Completion Status: QUESTION 6 Most analytical machines used in science can either measure absorbance of light, like the spectrophotometers used in BioZ 151 and 152 labs, or can measure change in electrical conductance. In order to convert things like absorbance values into concentration data, a linear regression is used. In order to determine nitrite concentration in water samples, nitrite is reacted with several chemicals to produce a purple color. The absorbance of the solution is measured from known amounts of nitrite to produce a standard curve to analyze samples. Use the data in the provided table, or from the linked spreadsheet to make a graph and perform a linear regression using treadline. mg/L NO, Absorbance 0.1 0.183 0.3 0.5 0.7 0.542 0.914 1.298 1.61 In order for this to work, samples must be diluted prior to measurement standard curve. If a sample underwent a 0.02 dilution, and produce an absorbance value of 0.903, what is the resultant concentration of NO2 in mg…
The protein content of the unknown samples were determined using BSA as standard. The absorbance values of the standard solutions and samples are as follows. BSA concentration (mg/mL) Absorbance 0.000 20 0.110 40 0.192 60 0.248 80 0.325 100 0.400 120 0.460 a. What is the equation of the line? b. Determine the protein concentration of samples in ug/mL. Sample dilutions 1 in 14 A540 0.150 1 in 5 1 in 8 0.380 0.235
q42 please calculate the unknown concentration of the protein D wih an absorbance value of A554 given the standard curve indicated in the table. write your answers in numbers only with 2 decimals. protein concentration (ug/ml) absorbance 0 0.000 0.02 0.161 0.04 0.284 0.06 0.438 0.08 0.572 0.10 0.762
A 0.1 g amine-containing compound is dissolved in water then diluted to 100 mL. To get the concentration of amine in this compound, you subject it to spectroscopic analysis. So, you get 1 mL of the previously diluted sample then dilute it again to 250 mL for measurement. Then, you fill 3/4 of a 1-cm cuvette with this diluted sample then you run an analysis using an AAS. The recorded absorbance is 0.545 at 410 nm. Solve for the molecular weight of the compound. The molar absorptivity is 1.23 x 104 cm-1 mol-1 L.
QUESTION 1 You obtained the following raw data when setting up a Bkuret standard curve BSA (mg/ml) Absorbancy 540nm 0.158 0210 0.260 3 0 305 4. 0360 0.410 6 0 455 0.510 0 530 0.550 10 0 554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.324 was obtained Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / mil (up to 3 significant figures).