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Match the following Conjugation terms with the most appropriate description in the image:
F- recombinant
F- strain
F+ strain
HFR strain
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- Three pairs of bacterial cells with the given genotypes undergo conjugation. Place match the genotype of each cell after conjugation to its initial genotype. F+ x F Hfr F- F' F- Answer Bank F F F+ Hfr What is the role of the F-factor in conjugation? It contains genes necessary for replication of the donor's F plasmid. can occur. It allows auxotrophic bacterial cells to survive on minimal medium so that conjugation It contains genes that force recombination between the donor and recipient chromosomes. It contains genes necessary for the formation of the pilus. O It degrades the chromosome of the recipient cell after conjugation.The ability of F+ cells, or Hfr cells, to transfer plasmid DNA to an F- cell is properly called: transversion transformation conjugation transduction transitionChoose the statement below that is true about the characteristics of the bacterial fertility factor factor. The fertility factor carries genes responsible for uptake of naked DNA, and has several IS elements to assist plasmid integration into host cell's chromosome. O The fertility factor carries genes responsible for formation of bacteriophages, and has several IS elements to assist plasmid transfer through the sex pilus. The fertlity factor carries genes responsible for formation of sex pili, and has several IS elements to assist plasmid integration into host cell's chromosome. The fertility factor carries genes responsible for formation of sex pili, and has several IS elements to assist plasmid transfer through the sex pilus.
- Jm109 cells transformed with ligation and plasmid alone is used. The colonies most like: white blue light blue No coloniesIn the genotype presented (genomic plus plasmid genes), which of the following statements Is phenotype? genome: IS P O`z+Y° plasmid: I* Pt otzY+ no production of either B-gal and permease O B-gal and permease induced when lactose is present no B-gal is produced and expression of permease when lactose is present constitutive expression of B-gal and permease constitutive expression of B-gal and no expression of permeaseOne feature that virtually all plasmid vectors have incommon is the polylinker (also called a multiple cloningsite). Explain what a polylinker is and why it is such animportant feature
- A cloning vector map is shown below. EcoRI Bam Ban Hind P-galactosidase Amp Bam Bam EcoRI Ori C Which restriction site is best for inserting a DNA fragment for selection of chimeric plasmid containing colonies? 1) They're all equally good. 2) Hindll 3) EcoRI 4) BamHIWhich of the following statements are TRUE? (Multiple Select - choose ALL that are TRUE) A bacterial cell has all of the genes necessary to generate a pillus, it just needs activation by an F plasmid O After conjugation, the cell that started out F- becomes F+ O Conjugation cannot occur between an F+ cell and an R+ cell O Conjugation cannot occur between an F+ cell and an F- cell Conjugation can occur between two strains even if the two strains are blocked from touching by a filter. O Most antibiotics block bacterial conjugation O After conjugation, the cell that started out F+ becomes F- O Conjugation cannot occur between an F+ cell and an F+ cellAn ade+ arg+ cys+ his+ leu+ pro+ bacterial strain is knownto be lysogenic for a newly discovered phage, but the siteof the prophage is not known. The bacterial map isleucysarghisadeproThe lysogenic strain is used as a source of the phage, andthe phages are added to a bacterial strain of genotypeade- arg- cys- his- leu- pro-. After a short incubation,samples of these bacteria are plated on six differentmedia, with the supplementations indicated in thefollowing table. The table also shows whether colonieswere observed on the various media.PresenceMedium Ade Arg Cys His Leu Pro of colonies1 - + + + + + N2 + - + + + + N3 + + - + + + C4 + + + - + + N5 + + + + - + C6 + + + + + - NNutrient supplementation in medium(In this table, a plus sign indicates the presence of anutrient supplement, a minus sign indicates that asupplement is not present, N indicates no colonies, and Cindicates colonies present.)a. What genetic process is at work here?b. What is the approximate locus of the prophage?
- An Hfr strain that is hisE+ and pheA+ was mixed with a strain thatis hisE− and pheA−. The conjugation was interrupted and the percentageof recombinants for each gene was determined by streakingon a medium that lacked either histidine or phenylalanine. Thefollowing results were obtained:It was found that hisE is 4 minutes away from pabB. The gene pheA was shown to be 17 minutes from pabB. Draw a genetic map describing the locations of all three genes.Describe the relevant features of the F factor (plasmid) that allow it to partake in conjugation.Contrast the use of plasmid vectors with that of BAC orYAC