10. Modify the diagrams below to reflect the activation and repression of lac operon. Usearrows, lines, X's, etc. to illustrate control of gene expression.Sa. ActivationlactoselactoseCAPlac Irepressorallolactoseb. RepressionCAPallolactoselac IrepressorCAMPсCAMPсpromoter operator+1RNAPol+1RNAPolpromoter operatorlac Zlac Zlac Ylac Ylac Alac ADNADNA

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Description: 10. Modify the diagrams below to reflect the activation and repression of lac operon. Usearrows, lines, X's, etc. to illustrate control of gene expression.Sa. ActivationlactoselactoseCAPlac Irepressorallolactoseb. RepressionCAPallolactoselac Irepres

Lac operon consists of the cluster of genes. The letters depicted in the lac operon below are as…

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Biology

lactose a. Activation CAP lac I repressor allolactose b. Repression CAP lac I repressor CAMP с CAMP с RNA Pol promoter operator RNA Pol promoter operator +1 lac Z lac Z lac Y lac Y lac A lac A DNA DN.

lactose a. Activation CAP lac I repressor allolactose b. Repression CAP lac I repressor CAMP с CAMP с RNA Pol promoter operator RNA Pol promoter operator +1 lac Z lac Z lac Y lac Y lac A lac A DNA DN.

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 9TYU: A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous...

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1. Draw a diagram of the lac operon. PO I+P+O+Z+Y+ I+P+OcZ+Y- I-P+O+Z+Y+ Lab section (circle): lacz gene lac Y gene lacA gene I+P+O+ZY/IPO+Z+Y- 2. The promoter (P) is the start site of transcription through the binding of RNA polymerase before actual mRNA production. Mutated promoters (P) cannot bind RNA polymerase, so no mRNA is made. In the table below, make predictions about the expression of ß-galactosidase and permease for different partial diploid (F') genotypes. Do this for E. coli growth on two different types of media - one without lactose, and one with. Use a to mark where there is no enzyme produced, and a + to mark where the enzyme is made. The first genotype has all wild-type alleles, and has been filled in as an example. Genotype IPOCZ+Y/I-P+O+Z-Y+ ISP+O+Z+Y/I+P+O+Z*Y* CR 1:00 Lactose absent B-galactosidase Permease R 3:-00 + R 6:30 Lactose present B-galactosidase F 10:00 Permease
Part A If E. coll bacteria are placed in an environment that contains both glucose and lactose, the regulation of the lac operon will allow which nutrient to be processed first? ONether will be processed in this environment O Both will be processed at the same time. Glucose O Not enough is known about the regulation of the lac operon to know which nutrient will be processed first Lactose Submit Request Answer
Answer as Directed. Below is the model of a lac operon. lac I lac Z с promoter operator +1 lac Y lac A DNA 1. In the absence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac control region? Is the operon being transcribed then? 2. In the presence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac regulatory region? Is the operon being transcribed then? 3. In the presence of lactose and the absence of glucose in the bacterial growth media, what proteins are bound to the lac control region? 4. Why is it adaptive for a bacterium to not express the genes that encode for that lactose utilization proteins when lactose is not available or when glucose is present? 5. Why is it adaptive for the structural genes for using lactose to be under the control of a single promoter, i.e., synthesize a polycistronic message rather than three monocistronic message?
The molecules indicated below can be used experimentally due to their roles in relation to the expression of the lac operon. Drag the listed molecules to their corresponding bin. Reset Help ONPG IPTG X-Gal allolactose Dragable beta-galactosidase cleaves this molecule to form a visible yellow product. beta-galactosidase cleaves this molecule which then dimerizes to form a visible blue product. This molecule induces the transcription of the lac operon by inactivating the lac repressor.
Answer as Directed. Below is the model of a lac operon. lac I lac Z с promoter operator lac Y lac A DNA +1 1. What are structural genes? Are the lac structural genes transcribed in the absence of lactose? 2. What is the role of the promoter and operator sites in the operon? 3. Is the repressor protein bound to the operator site in the absence of lactose? In its absence? 4. Under what nutritional circumstances (high or low glucose) is CAP bound to cAMP? 5. In the absence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac control region? Is the operon being transcribed then? 6. In the presence of lactose and the presence of glucose in the bacterial growth media, what proteins are bound to the lac regulatory region? Is the operon being transcribed then? 7. In the presence of lactose and the absence of glucose in the bacterial growth media, what proteins are bound to the lac control region? 8. Why is it adaptive for a bacterium to not…
Select the correct statement about the trp operon: high levels of tryptophan promotes high transcription of CAP O low levels of tryptophan promote transcription of trp operon genes O high levels of tryptophan stall ribosomes to reduce tryptophan synthesis O high levels of tryptophan promote transcription of trp operon genes
Match each description with the appropriate label (allo)Lactose binds to the lac repressor, causing it to release from the promoter CAP binds CAMP to stimulate transcripti of the lac operon ✓ [Choose ] positive control of an inducible gene negative control of an inducible gene [Choose]
In the presence of lactose and the presence of glucose the lac operon is V [ Select ] Lacl is [ Select ] t. expressed at basal levels strongly expressed to the CAP binding site. not expressed
12a. In what condition is the LacZ gene transcribed? ANSWER (in one sentence) 12b. Fill in the following table about the Lac operon (LacZ gene) Environmental Condition + Glucose and + Lactose Glucose and + Lactose - Glucose and - Lactose + Glucose and - Lactose Q 2 Body" W #3 E D Which proteins bind to the LacZ promoter? ANSWER ANSWER ANSWER ANSWER $ 4 A R F B % 5 I T MacBook Pro A U 6 G Y & 7 H Is LacZ transcribed? (Yes or No) ANSWER ANSWER ANSWER ANSWER |||| U 8 K
Read aloud V Draw Highlight 2. You are studying the regulation of the lactose operon in Escherichia coli, by measuring expression of the lacZ gene (i.e production of beta-galactosidase). (a) You identify several loss-of-function mutations in which lacZ is never expressed, in the presence and absence of glucose and lactose. What components of the lac operon could be mutated to produce this phenotype? List all possibilities. (b) You identify another loss-of-function mutation with the following expression pattern: Media + glucose - lactose + glucose +lactose - glucose - lactose - glucose + lactose lacZ expression Low Low High High What components of the lac operon could be mutated to produce this phenotype? List all possibilities.
Indicate whether each of the following statements about the lac operon are true or false: Bacteria with a lac phenotype are unable to use lactose because of some defect in expressing the genes A constitutive Lac+ phenotype could mean either that the production of repressor is defective or that the operator sequence is defective The lacl gene is next to, but is not part of, the lac 1. TRUE operon 2. FALSE Bacteria with a lac* phenotype cannot grow on media containing lactose as the only sugar The structural genes of the lac operon are each transcribed as a separate MRNA Expression of the Lac Repressor from the lacl promoter is regulated by lactose
1. Complete the following table based on your lac operon simulations. Absence of lactose Presence of lactose B-galactosidase permease B-galactosidase permease I*P*O*Z*Y* Mututed ISP+O*ZY* I'P*O°Z*Y* I*P*O°ZY