Lab Day & Time - Negative End Marker Crime Suspect Suspect Standard Scene # 1 # 2 wells og aib d to eadm + Positive End 1. When you simply look at the banding patterns on the gel from the DNA fingerprint we ran in lab as seen above, which two profiles match? 2. Does the fact that one suspect's DNA fingerprint matches the DNA at the crime scene prove their guilt? Why or why not? 3. The DNA was originally placed in the wells of the gel near the negative end of the gel and fragments moved towards the positive end of the gel. What charge do DNA molecules have? Positive II|| | |

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Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa
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Chapter29: Examinations And Procedures Of Body Systems
Section: Chapter Questions
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Worksheet for Lab 12: DNA FINGERPRINTING
Name
Lab Day & Time
- Negative End
Marker Crime Suspect Suspect
Standard Scene
# 1
# 2
wells
to eadms d al ib mot
+ Positive End
nian AMa hos yb
1. When you simply look at the banding patterns on the gel from the DNA fingerprint we ran in
lab as seen above, which two profiles match?
2. Does the fact that one suspect's DNA fingerprint matches the DNA at the crime scene prove
their guilt? Why or why not?
3. The DNA was originally placed in the wells of the gel near the negative end of the gel and
fragments moved towards the positive end of the gel. What charge do DNA molecules have?
Positive
4a. Where on the gel are the longest fragments found?
de gel, on
lectrode
4b. Where on the gel are the shortest fragments found?
IX
che samples is mixeu w
the sample to sink to the bottom of the wen.
ally followed as it moves down the gel. TE buffer is matched to the gef TO assure-
- bottom of the wells with the micropipette tip.
tips contaminated with DNA in the special disposal container.
? (It gives
is dry, place it onto the electrophoresis chamber. Wipe off any spills on the
0 the next step.
II|| |
Transcribed Image Text:Worksheet for Lab 12: DNA FINGERPRINTING Name Lab Day & Time - Negative End Marker Crime Suspect Suspect Standard Scene # 1 # 2 wells to eadms d al ib mot + Positive End nian AMa hos yb 1. When you simply look at the banding patterns on the gel from the DNA fingerprint we ran in lab as seen above, which two profiles match? 2. Does the fact that one suspect's DNA fingerprint matches the DNA at the crime scene prove their guilt? Why or why not? 3. The DNA was originally placed in the wells of the gel near the negative end of the gel and fragments moved towards the positive end of the gel. What charge do DNA molecules have? Positive 4a. Where on the gel are the longest fragments found? de gel, on lectrode 4b. Where on the gel are the shortest fragments found? IX che samples is mixeu w the sample to sink to the bottom of the wen. ally followed as it moves down the gel. TE buffer is matched to the gef TO assure- - bottom of the wells with the micropipette tip. tips contaminated with DNA in the special disposal container. ? (It gives is dry, place it onto the electrophoresis chamber. Wipe off any spills on the 0 the next step. II|| |
5. What would have happened if the gel were placed backwards in the electrophoresis chamber
with the wells containing the DNA near the positive end of the gel?
omiT 3 vea dsJ
bn ovitegoM-
6a. How many DNA fragments were in Suspect #1's sample?
6b. How many DNA fragments were in Suspect #2°s sample?
7. Suspect #1 and Suspect #2 have different numbers of fragments. Explain why DNA samples
from different people can have different numbers of fragments.
8. Explain the difference between tracking dye and DNA stain.
bnTovilleo+
ad od
ods
Transcribed Image Text:5. What would have happened if the gel were placed backwards in the electrophoresis chamber with the wells containing the DNA near the positive end of the gel? omiT 3 vea dsJ bn ovitegoM- 6a. How many DNA fragments were in Suspect #1's sample? 6b. How many DNA fragments were in Suspect #2°s sample? 7. Suspect #1 and Suspect #2 have different numbers of fragments. Explain why DNA samples from different people can have different numbers of fragments. 8. Explain the difference between tracking dye and DNA stain. bnTovilleo+ ad od ods
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