in HPLC, what does it mean to equilibrate the system. Let us assume that the system is currently running 100%B. You have told them to run the first standard of caffeine using Reverse Phase chromatography with a C18 column, 30% A (Water/0.1%TFA) and 70% B (MeOH/0.1%TFA). What should I equilibrate the system to and for how long?

in HPLC, what does it mean to equilibrate the system. Let us assume that the system is currently running 100%B. You have told them to run the first standard of caffeine using Reverse Phase chromatography with a C18 column, 30% A (Water/0.1%TFA) and 70% B (MeOH/0.1%TFA). What should I equilibrate the system to and for how long?

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter88: Column Chromatography

Section: Chapter Questions

Problem 5P

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