A. THIN LAYER CHROMATOGRAPHY Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubia tinctorum, commonly known as rose madder. Earth spotted the root extract on two hydrophobic silica gel plates. He then placed each plate in separate developing chambers, one containing solvent system WHITE and the other housing solvent system MIX. Earth allowed the chromatograms to develop for around five minutes. Afterwards, he removed the plates from the containers and placed them inside an iodine chamber. The chromatograms resulting from the TLC experiment after visualization are illustrated as follows. The distances travelled by each spot and by the solvents were measured and recorded in the displayed table. D 75.0 mm 71.5 mm DISTANCE SOLVENT SPOTS TRAVELLED FROM SYSTEM solvent THE ORIGIN, mm front A 13.0 В 32.5 WHITE 41.0 D 66.5 A 10.5 B 27.5 MIX A - origin, A 39.0 D 53.5 10.5 mm 8 mm WHITE MIX O mm- O mm
A. THIN LAYER CHROMATOGRAPHY Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubia tinctorum, commonly known as rose madder. Earth spotted the root extract on two hydrophobic silica gel plates. He then placed each plate in separate developing chambers, one containing solvent system WHITE and the other housing solvent system MIX. Earth allowed the chromatograms to develop for around five minutes. Afterwards, he removed the plates from the containers and placed them inside an iodine chamber. The chromatograms resulting from the TLC experiment after visualization are illustrated as follows. The distances travelled by each spot and by the solvents were measured and recorded in the displayed table. D 75.0 mm 71.5 mm DISTANCE SOLVENT SPOTS TRAVELLED FROM SYSTEM solvent THE ORIGIN, mm front A 13.0 В 32.5 WHITE 41.0 D 66.5 A 10.5 B 27.5 MIX A - origin, A 39.0 D 53.5 10.5 mm 8 mm WHITE MIX O mm- O mm
Chapter14: Chromatography
Section: Chapter Questions
Problem 9P
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1. Solve for the Rf values of the spots formed for each solvent. Report your answers in four decimal places.
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