Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…
A: Serial dilution is a progression of successive dilutions used to lessen a thick culture of cells to…
Q: Two different bacterial samples, A and B, were inoculated onto SIM media. What can you conclude…
A: SIM (Sulfide, Indole and Motility) test is done to differentiate the Gram negative bacilli based on…
Q: What are the pros and cons of serial dilution method and W/V % method?
A:
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A: In laboratories, the bacterial cultured on the agar plates are counted by the help of CFU(colony…
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A: A spectrophotometer is an instrument that can determine the concentration of a compound or solution…
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A: the hanging drop method is better because it allow for longer time visualisation .
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A: A microbial culture made by transferring the cells from the previous/old culture medium into a fresh…
Q: What are the physical methods of microbial control
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A: Hemocytometer is a chambered device which is used to calculate the blood cells. It is widely used in…
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A: The organisms that can only be seen through the microscope, known as microorganisms. These include…
Q: Why are the tubes sealed with a rubber stopper rather than left open to the atmosphere?
A: A rubber stopper is used to seal the tube opening as well as other laboratory glassware such as…
Q: For an In vitro mimicking infection test:
A: Introduction: Cell culture is the process of growing cells in a controlled setting, usually outside…
Q: Differentiate between alpha- and beta-hemolysis.
A: Hemolysis is the rupturing or breakdown of red blood cells (RBCs) and the oxygen-carrying hemoglobin…
Q: how to calculate Calculate PFU -plaque forming units
A: PFU ( plaque forming units) is the Virus titer (Virus per ml). A plaque-forming unit (PFU) is a…
Q: You perform a series of 4 different dilutions of 200 µL of sample into 800 µL of saline. What is the…
A: Dilution factor can be calculated using the formula:C1V1=C2V2
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Q: How do a spectrophotometer analyze a blood sample ? How do you prepare the sample to be analysed ?
A: A spectrophotometer measures the degree to which a chemical substance is able to absorb light when…
Q: What is the principle of counting with a hemocytometer? What are the advantages and disadvantages?
A: Answer: HEMOCYTOMETER : It is the tool used in most of the biochemistry and biology labs in order to…
Q: How to use the sterile filter? Show the different sizes and specifications of sterile filters.
A: Sterilization is the method that is used to eliminate all forms of life and biological agents…
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A: The passage of water through a membrane is known as osmosis. The movement of water to the side of…
Q: I missed a couple of my labs due to covid and am struggling to teach myself how to do serial…
A: The last two years have been extremely difficult because you can still learn and understand the…
Q: Explain how to perform a serial dilution
A: Serial dilution is stepwise dilution of a solution keeping the dilution factor constant in each…
Q: What is the difference between in-situ & ex-situ conservation?
A: Conservation plays a major role in preserving animal and plant species that are in the verge of…
Q: Name any two conventional methods of ex-situ conservation?
A: Conservation plays a major role in preserving animal and plant species that are in the verge of…
Q: Describe three problems associated with using the standard plate count method for determining the…
A: Typically, an accurate count of bacterial colonies is required for biological processes. Microbial…
Q: Chromatography technique works on the principle of
A: Chromatography is a technique used for separation of mixture by using a moving solvent.
Q: How could hemophiliacs benefit from affinity affinity chromatography?
A: Hemophilia shows the X linked recessive pattern of inheritance.This disease mostly affected the…
Q: who introduced the use of cotton plug in microbial culture tubes.
A: Cotton plugs are utilized in bacterial specimens and culture mediums. The cotton plugs' primary role…
Q: What is meant by recalcitrance in microbiology? Give an example
A: Now a days environmental pollution is one of the major issue and it is defined as any undesirable…
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A: A lysis buffer is a buffer solution used for the purpose of breaking open cells for use in molecular…
Q: Design a serial dilution procedure to achieve a 56-colony count, from a sample with 8.75x105 CFU/mL…
A: Serial Dilution -- Serial dilution are used to calculate the concentration of microorganism .It…
Q: A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it.…
A: A serial dilution is a process of sequential dilution in order to decrease the density of cell…
Q: List down two important precautions in conducting brine solution experiment.
A: Brine solution experiment : The brine solution experiment is generally used to study the process of…
Q: What could be possible errors when you incorrectly identify an Unknown organism when using IMViC…
A: IMViC tests are used to identify bacteria from specimens collected from patients in majority of…
Q: The wavelength we used in hemoglobin determination experiment is: 700 nm. O b. 350 nm, C. 540 m. O…
A: Hemoglobin is an iron-containing oxygen-transport globular protein found in virtually all mammals'…
Q: Based on these blood agar results, can you identify what type of hemolysis are occurring in these…
A: Hemolysis is the destruction of red blood cells. It can occur due to different causes and leads to…
Q: What are the main types of immobilization techniques used in biosensors? (with a deep explanation)
A: Immobilization It is a technique that is used for the chemical and physical fixation of the…
Q: Neubauer haemocytometer
A: Neubauer haematocytometer is the most common method for cell counting in the world used by…
Q: Why is it important that you should know the laboratory apparatuses and reagents as a future medical…
A: LABORATORY APPARATUS- The many devices and equipment used by scientists working in a laboratory…
Q: Define the term sterile and explain why sterile instruments and media are necessary to be used in…
A: Microbes or microorganisms are very very small organisms that are visible under the microscope. They…
Q: Provide information about the hemocytometer. Explain the principle of counting with hemocytometer…
A: The hemocytometer is a specimen slide which is used to determine the concentration of cells in a…
Q: What is the purpose of flaming an inoculating loop?
A: The question asks to give the reason for flaming or sterilizing an inoculating loop.
Q: What are two advantages of filtration in microbial control? Explain how the filtration process…
A: According to the question, we have to describe two advantages of filtration in microbial control. In…
Q: Define the importance of minimal medium in biology ?
A: A growth medium, also known as a culture media, is a solid, liquid, or semi-solid medium that is…
Given the widespread automation, why is it critical that we understand the principle of the hemocytometer and how to use it?
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- What is the principle of counting with a hemocytometer? What are the advantages and disadvantages?I need assistance with how to read Fletcher Munson graphs. Please help and show me the proper way to analyze these graphs. The previous experts I've asked on this platform were incorrect.explain why it is important to maintain sterility when inserting a foley cathether
- Using the data in the photo of the two syringes below, answer these six questions: QUESTIONS 1. Which syringe had the most floating disks? Why? 2. What is the gas being released by the leaf disks? 3. Which syringe is the control? 4. Which syringe is the experimental? 5. What is the experimental variable in this experimental design? 6. List at least two controls in this experimental design.Using the date in the photo of the two syringes below, answer these six questions: QUESTIONS 1. Which syringe had the most floating disks? Why? 2. What is the gas being released by the leaf disks? 3. Which syringe is the control? 4. Which syringe is the experimental? 5. What is the experimental variable in this experimental design? 6. List at least two controls in this experimental design.Briefly explain the principle behind flow cytometry What is/are purpose of flow cytometry?
- A flow cytometer is constructed of many components. For good quantitative results how and how frequently should the various systems be calibrated? What is MESF and how does it apply in flow cytometry?What are the principles of pulse oximetry technology?State in details, what are the sources of error that could impact your data and interpretation when using the “ Neubauer haemocytometer” technique.
- What are the readings that are displayed on the screen of the semi automated analyzer?Why is the micropipette an important tool in the clinical chemistry section?A nurse who created a sterile field for a patient is adding asterile solution to the field. What is an appropriate actionwhen performing this task?a. Place the bottle cap on the table with the edges down.b. Hold the bottle inside the edge of the sterile field.c. Hold the bottle with the label side opposite the palm ofthe hand.d. Pour the solution from a height of 4 to 6 inches (10 to15 cm).
